Construct: ORF TRCN0000469040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010453.1_s317c1
- Derived from:
- ccsbBroadEn_08429
- DNA Barcode:
- TCAATTTCCGCTGGGTTATCTCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SPDL1 (54908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469040
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_001329641.2 | 99.7% | 99.3% | (many diffs) |
| 2 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_017785.5 | 99.7% | 99.3% | (many diffs) |
| 3 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_001329639.2 | 88.7% | 89.4% | (many diffs) |
| 4 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_001329640.2 | 88.7% | 89.4% | (many diffs) |
| 5 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | XM_011534587.2 | 88.7% | 89.4% | (many diffs) |
| 6 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | XM_011534588.2 | 88.7% | 89.4% | (many diffs) |
| 7 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | XM_011534589.2 | 88.7% | 89.4% | (many diffs) |
| 8 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_001329643.2 | 88% | 87.6% | (many diffs) |
| 9 | human | 54908 | SPDL1 | spindle apparatus coiled-co... | NM_001329642.2 | 77.7% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1875
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcagatata atcacaaatc ttcgatgcag gctcaaagag gctgaagaag 121 agcgactaaa agctgcacag tatggtttac aactagtaga gagtcaaaat gaattacaga 181 atcaattgga taaatgtcgt aatgaaatga tgaccatgac tgagagttat gaacaagaaa 241 aatataccct tcaaagagaa gttgaactca agagtcgaat gttagaaagt ttgagctgcg 301 aatgtgaagc tattaaacaa caacaaaaaa tgcacctgga gaaattggaa gaacaactaa 361 gcagaagcca tggacaggaa gtgaatgaac taaaaactaa gatagaaaaa ctgaaagtgg 421 aattagatga agccaggctt agtgaaaagc agctgaagca ccaagtagat catcagaagg 481 aactcctctc ttgtaaatca gaggaactgc gcgtaatgtc tgaacgtgtg caggaaagca 541 tgtcttcaga gatgctggct cttcaaattg agctgacaga aatggagagt atgaagacca 601 ccctcaaaga agaagtgaat gaactacaat acagacaaga acagctagaa cttcttatta 661 ctaacctaat gcgccaggta gaccggctta aagaggaaaa agaggagcga gagaaagaag 721 cagtttctta ctataatgcc ctagagaaag ctcgtgtagc aaatcaagat cttcaggtac 781 agttggacca ggcactccag caagccttgg atcccaatag taaaggcaac tctttgtttg 841 cagaggtgga agatcgaagg gcagcaatgg aacgtcagct tatcagtatg aaagtcaagt 901 atcagtcact aaagaagcaa aatgtattta acagagaaca gatgcagaga atgaagttac 961 aaattgccac gttgctacag atgaaagggt ctcaaactga atttgagcag caggaacggt 1021 tgcttgccat gttggagcag aagaatggtg aaataaaaca tcttttaggt gaaattagaa 1081 atctggagaa atttaagaat ttatatgaca gtatggaatc taagccttca gtcgactctg 1141 gtactctgga agataacacc tattatacag atttacttca gatgaagctg gataacttaa 1201 acaaagaaat tgaaagcact aaaggtgaat tgtccataca gcgaatgaaa gcattatttg 1261 agagccagcg ggctctagat attgagcgaa aactttttgc aaatgaaaga tgcctccagc 1321 tttcagaaag tgaaaatatg aaactgagag ctaaactaga tgaattgaaa ctaaaatatg 1381 aacctgaaga gacagttgaa gtgcctgtac tgaaaaagag gcgtgaggtg ctccctgtgg 1441 atataaCCAC CGCTAAAGAT GCATGTGTCA ACAACAGTGC TCTCGGGGGA GAAGTTTATC 1501 GATTACCGCC TCAGAAAGAG GAGACACAGT CCTGCCCTAA CAGTTTAGAA GATAACAACT 1561 TGCAATTAGA AAAATCAGTT TCTATACACA CACCAGTAGT CAGTCTCTCT CCTCACAAAA 1621 ATCTGCCCGT GGATATGCAG CTGAAGAAGG AAAAGAAATG TGTGAAACTC ATAGGAGTTC 1681 CCGCTGACGC TGAGGCCTTA AGTGAAAGAA GTGGAAACAC CCCTAACTCT CCCAGGTTAG 1741 CTGCTGAATC AAAGCTTCAA ACAGAAGTTA AAGAAGGAAA AGAAACTTCA AGCAAATTGG 1801 AAAAAGAAAC TTGTAAGAAA TCACACCCTA TTCTATATGT GTCTTCTAAA TCTACTCCAG 1861 AGACCCAGTG CCCTTAACAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAA TTTCCGCTGG 2041 GTTATCTCAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt