Construct: ORF TRCN0000469043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015504.1_s317c1
- Derived from:
- ccsbBroadEn_11353
- DNA Barcode:
- CCACTTATCCCCGACCGATTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUMBL (9253)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9253 | NUMBL | NUMB like endocytic adaptor... | NM_001289979.2 | 100% | 100% | |
| 2 | human | 9253 | NUMBL | NUMB like endocytic adaptor... | NM_001289980.2 | 100% | 100% | |
| 3 | human | 9253 | NUMBL | NUMB like endocytic adaptor... | NM_004756.5 | 93.2% | 93.2% | 1_123del |
| 4 | mouse | 18223 | Numbl | numb-like | XM_006539617.1 | 88.6% | 95.7% | (many diffs) |
| 5 | mouse | 18223 | Numbl | numb-like | NM_010950.2 | 82.7% | 89.3% | (many diffs) |
| 6 | mouse | 18223 | Numbl | numb-like | XM_006539615.3 | 82.1% | 88.7% | (many diffs) |
| 7 | mouse | 18223 | Numbl | numb-like | XM_006539616.1 | 82.1% | 88.7% | (many diffs) |
| 8 | mouse | 18223 | Numbl | numb-like | XM_011250452.1 | 74.5% | 80.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1770
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caagttacgg cagagcctgc ggcggaggaa gccagcctac gtgcccgagg 121 cgtcgcgccc gcaccagtgg caggcagacg aggacgcggt gcggaagggc acgtgcagct 181 tcccggtcag gtacctgggt cacgtggagg tagaggagtc ccggggaatg cacgtgtgtg 241 aagatgcggt gaagaagctg aaggcgatgg gccgaaagtc cgtgaagtct gtcctgtggg 301 tgtcagccga tgggctccga gtggtggacg acaaaaccaa ggatcttctg gtcgaccaga 361 ccatcgaaaa ggtctccttt tgtgctcctg accgcaacct ggacaaggct ttctcctata 421 tctgtcgtga cgggactacc cgccgctgga tctgccactg ttttctggca ctgaaggact 481 ccggcgagag gctgagccac gctgtgggct gtgcttttgc cgcctgcctg gagcgaaaac 541 agcgacggga gaaggaatgt ggggtcacgg ccgccttcga tgccagccgc accagcttcg 601 cccgcgaggg ctccttccgc ctgtctgggg gtgggcggcc tgctgagcga gaggccccgg 661 acaagaagaa agcagaggca gcagctgccc ccactgtggc tcctggccct gcccagcctg 721 ggcacgtgtc cccgacacca gccaccacat cccctggtga gaagggtgag gcaggcaccc 781 ctgtggctgc aggcaccact gcggccgcca tcccccggcg ccatgcaccc ctggagcagc 841 tggttcgcca gggctccttc cgtgggttcc cagcactcag ccagaagaac tcgcctttca 901 aacggcagct gagcctacgg ctgaatgagc tgccatccac gctgcagcgc cgcactgact 961 tccaggtgaa gggcacagtg cctgagatgg agcctcctgg tgccggcgac agtgacagca 1021 tcaacgctct gtgcacacag atcagttcat cttttgccag tgctggagcg ccagcaccag 1081 ggccaccacc tgccacaaca gggacttctg cctggggtga gccctccgtg ccccctgcag 1141 ctgccttcca gcctgggcac aagcggacac cttcagaggc tgagcgatgg ctggaggagg 1201 tgtcacaggt ggccaaggcc cagcagcagc agcagcagca acagcaacag cagcagcagc 1261 agcagcagca acagcagcaa gcagcctcag tggccccagt gcccaccatg cctcctgccc 1321 tgcagccttt ccccgccccc gtggggCCCT TTGACGCTGC ACCTGCCCAA GTGGCCGTGT 1381 TCCTGCCACC CCCACACATG CAGCCCCCTT TTGTGCCCGC CTACCCGGGC TTGGGCTACC 1441 CACCGATGCC CCGGGTGCCC GTGGTGGGCA TCACACCCTC ACAGATGGTG GCAAACGCCT 1501 TCTGCTCAGC CGCCCAGCTC CAGCCTCAGC CTGCCACTCT GCTTGGGAAA GCTGGGGCCT 1561 TCCCGCCCCC TGCCATACCC AGTGCCCCTG GGAGCCAGGC CCGCCCTCGC CCCAATGGGG 1621 CCCCCTGGCC CCCTGAGCCA GCGCCTGCCC CAGCTCCAGA GTTGGACCCC TTTGAGGCCC 1681 AGTGGGCGGC ATTAGAAGGC AAAGCCACTG TAGAGAAACC CTCCAACCCC TTTTCTGGCG 1741 ACCTGCAAAA GACATTCGAG ATTGAACTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAGCTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCACT 1921 TATCCCCGAC CGATTCTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt