Construct: ORF TRCN0000469044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015882.1_s317c1
- Derived from:
- ccsbBroadEn_02456
- DNA Barcode:
- CCTTAACCTGCATGCTCCTATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VTI1B (10490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10490 | VTI1B | vesicle transport through i... | NM_006370.3 | 100% | 100% | |
| 2 | mouse | 53612 | Vti1b | vesicle transport through i... | NM_016800.3 | 89% | 92.6% | (many diffs) |
| 3 | mouse | 53612 | Vti1b | vesicle transport through i... | XM_006516088.3 | 64.7% | 67.6% | (many diffs) |
| 4 | mouse | 53612 | Vti1b | vesicle transport through i... | XM_011244143.1 | 64.7% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcctccgcc gcctcctcgg agcatttcga gaagctgcac gagatcttcc 121 gcggcctcca tgaagaccta caaggggtgc ccgagcggct gctggggacg gcggggaccg 181 aagaaaagaa gaaattgatc agggattttg atgaaaagca acaggaagca aatgaaacgc 241 tggcagagat ggaggaggag ctacgttatg cacccctgtc tttccgaaac cccatgatgt 301 ctaagcttcg aaactaccgg aaggaccttg ctaaactcca tcgggaggtg agaagcacac 361 cttTGACAGC CACACCTGGA GGCCGAGGAG ACATGAAATA TGGCATATAT GCTGTAGAGA 421 ATGAGCATAT GAATCGGCTA CAGTCTCAAA GGGCAATGCT TCTGCAGGGC ACTGAAAGCC 481 TGAACCGGGC CACCCAAAGT ATTGAACGTT CTCATCGGAT TGCCACAGAG ACTGACCAGA 541 TTGGCTCAGA AATCATAGAA GAGCTGGGGG AACAACGAGA CCAGTTAGAA CGTACCAAGA 601 GTAGACTGGT AAACACAAGT GAAAACTTGA GCAAAAGTCG GAAGATTCTC CGTTCAATGT 661 CCAGAAAAGT GACAACCAAC AAGCTGCTGC TTTCCATTAT CATCTTACTG GAGCTCGCCA 721 TCCTGGGAGG CCTGGTTTAC TACAAATTCT TTCGCAGCCA TTGCCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGACCT TAACCTGCAT GCTCCTATAG GACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t