Construct: ORF TRCN0000469047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005675.1_s317c1
- Derived from:
- ccsbBroadEn_11515
- DNA Barcode:
- TGGCACCAGCCAGTGGTTTCCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOP56 (10528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10528 | NOP56 | NOP56 ribonucleoprotein | NM_006392.4 | 29.2% | 29.2% | 1_1260del |
| 2 | human | 10528 | NOP56 | NOP56 ribonucleoprotein | NR_145428.2 | 25.7% | 1_1289del;1448_1563del;1928_2029del | |
| 3 | human | 10528 | NOP56 | NOP56 ribonucleoprotein | NR_027700.3 | 24.9% | 1_1469del;1992_2093del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 ttggcatgaa ggaagcaatg gttcaggcag aggaagcggc tgctgagatt actaggaagc 121 tGGAGAAACA GGAGAAGAAA CGCTTAAAGA AGGAAAAGAA ACGGCTGGCT GCACTTGCCC 181 TCGCGTCTTC AGAAAACAGC AGTAGTACTC CAGAGGAGTG TGAGGAGATG AGTGAAAAAC 241 CCAAAAAGAA GAAAAAGCAA AAGCCCCAGG AGGTTCCTCA GGAGAATGGA ATGGAAGACC 301 CATCTATCTC TTTCTCCAAA CCCAAGAAAA AGAAATCTTT TTCCAAGGAG GAGTTGATGA 361 GTAGCGATCT TGAAGAGACC GCTGGCAGCA CCAGTATTCC CAAGAGGAAG AAGTCTACAC 421 CCAAGGAGGA AACAGTTAAT GACCCTGAGG AGGCAGGCCA CAGAAGTGGC TCCAAGAAAA 481 AGAGGAAATT CTCCAAAGAG GAGCCGGTCA GCAGTGGGCC TGAAGAGGCG GTTGGCAAGA 541 GCAGCTCCAA GAAGAAGAAA AAGTTCCATA AAGCATCCCA GGAAGATTAC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGATGGCACC AGCCAGTGGT TTCCGGCACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt