Construct: ORF TRCN0000469054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006564.1_s317c1
- Derived from:
- ccsbBroadEn_04494
- DNA Barcode:
- GCGCTTCTTTTGCATCCTGAGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A21 (89874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89874 | SLC25A21 | solute carrier family 25 me... | NM_030631.4 | 100% | 100% | |
| 2 | human | 89874 | SLC25A21 | solute carrier family 25 me... | NM_001171170.2 | 99.6% | 99.6% | 894_895insTGG |
| 3 | human | 89874 | SLC25A21 | solute carrier family 25 me... | XM_011537287.3 | 92.2% | 92.4% | (many diffs) |
| 4 | human | 89874 | SLC25A21 | solute carrier family 25 me... | XM_011537288.3 | 89.9% | 89.9% | 0_1ins90 |
| 5 | human | 89874 | SLC25A21 | solute carrier family 25 me... | XM_011537289.3 | 81.7% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 966
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgccaag cctgaagtca gcttagtgcg cgaggcttct cggcagatcg 121 tggccggtgg ttctgcaggt cttgtagaaa tttgcctgat gcacccccta gatgtggtga 181 aaaccaggtt tcagattcag agatgtgcaa ccgatccaaa cagttataaa agcttggtag 241 acagctttcg aatgattttc caaatggaag ggttatttgg tttttacaag ggaattctgc 301 cacctatctt ggctgaaacc ccaaaaagag cagtgaagtt tttcaccttt gagcagtaca 361 agaaattgct gggatatgtg tcactgtcac cagcattgac attcgccatt gctggattgg 421 gatctggact aacagaagcc attgtagtta acccttttga ggtagtaaaa gttggcttgc 481 aagcaaatcg gaacacattt gcagagcaac catccactgt gggttatgca agacaaatca 541 ttaagaagga aggctgggga ctccagggcc tcaacaaagg attaactgca actttgggac 601 gacatggagt tttcaacatg gtttattttg gcttcTACTA CAATGTCAAA AACATGATTC 661 CTGTCAATAA GGATCCAATC TTGGAGTTTT GGAGAAAATT TGGGATTGGT CTTCTCTCGG 721 GGACAATAGC CTCAGTCATT AACATCCCTT TTGATGTTGC CAAAAGTAGG ATTCAAGGGC 781 CTCAACCAGT TCCTGGAGAG ATCAAGTACA GAACCTGTTT TAAAACAATG GCAACAGTCT 841 ATCAGGAAGA AGGGATTTTA GCTTTGTACA AAGGCCTGCT TCCCAAGATT ATGAGACTTG 901 GACCAGGTGG TGCAGTGATG CTGCTGGTTT ATGAATACAC CTATTCATGG CTTCAAGAGA 961 ACTGGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGCA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCGCTTCTT TTGCATCCTG AGTTAACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt