Construct: ORF TRCN0000469055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009278.1_s317c1
- Derived from:
- ccsbBroadEn_01590
- DNA Barcode:
- TCTAGTGGATAAAGTCTAATGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRD5A2 (6716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469055
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6716 | SRD5A2 | steroid 5 alpha-reductase 2 | NM_000348.4 | 100% | 100% | |
| 2 | human | 6716 | SRD5A2 | steroid 5 alpha-reductase 2 | XM_011533069.2 | 69% | 63.9% | (many diffs) |
| 3 | human | 6716 | SRD5A2 | steroid 5 alpha-reductase 2 | XM_011533072.2 | 65.7% | 64.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 831
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaggttcag tgccagcaga gcccagtgct ggcaggcagc gccactttgg 121 tcgcccttgg ggcactggcc ttgtacgtcg cgaagccctc cggctacggg aagcacacgg 181 agagcctgaa gccggcggct acccgcctgc cagcccgcgc cgcctggttc ctgcaggagc 241 tgccttcctt cgcggtgccc gcggggatcc tcgcccggca gcccctctcc ctcttcgggc 301 cacctgggac ggtacttctg ggcctcttct gcctacatta cttccacagg acatttgtgt 361 actcactgct caatcgaggg aggccttatc cagctatact cattctcaga ggcactgcct 421 tctgcactgg aaatggagtc cttcaaggct actatctgat ttactgtgct gaataccctg 481 atgggtggta cacagacata cggtttagct tgggtgtctt cttatttatt ttggGAATGG 541 GAATAAACAT TCATAGTGAC TATATATTGC GCCAGCTCAG GAAGCCTGGA GAAATCAGCT 601 ACAGGATTCC ACAAGGTGGC TTGTTTACGT ATGTTTCTGG AGCCAATTTC CTCGGTGAGA 661 TCATTGAATG GATCGGCTAT GCCCTGGCCA CTTGGTCCCT CCCAGCACTT GCATTTGCAT 721 TTTTCTCACT TTGTTTCCTT GGGCTGCGAG CTTTTCACCA CCATAGGTTC TACCTCAAGA 781 TGTTTGAGGA CTACCCCAAA TCTCGGAAAG CCCTTATTCC ATTCATCTTT TTGCCAACTT 841 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 901 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 961 CTTGTGGAAA GGACGATCTA GTGGATAAAG TCTAATGCAA ACGCGTTAAG TCgacaatca 1021 acctctggat tacaaaattt gtgaaagatt