Construct: ORF TRCN0000469059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001280.1_s317c1
- Derived from:
- ccsbBroadEn_07675
- DNA Barcode:
- AAGGTAAAACCAGTCTTACAGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP50 (10762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10762 | NUP50 | nucleoporin 50 | NM_153645.2 | 99.8% | 100% | 111G>T;984T>C |
| 2 | human | 10762 | NUP50 | nucleoporin 50 | XM_005261312.1 | 99.8% | 100% | 111G>T;984T>C |
| 3 | human | 10762 | NUP50 | nucleoporin 50 | XM_005261314.1 | 99.8% | 100% | 111G>T;984T>C |
| 4 | human | 10762 | NUP50 | nucleoporin 50 | XM_006724104.1 | 99.8% | 100% | 111G>T;984T>C |
| 5 | human | 10762 | NUP50 | nucleoporin 50 | XM_017028537.2 | 99.8% | 100% | 111G>T;984T>C |
| 6 | human | 10762 | NUP50 | nucleoporin 50 | NM_007172.4 | 93.8% | 94% | 1_84del;195G>T;1068T>C |
| 7 | human | 10762 | NUP50 | nucleoporin 50 | XM_006724103.1 | 93.8% | 94% | 1_84del;195G>T;1068T>C |
| 8 | human | 10762 | NUP50 | nucleoporin 50 | XM_011529833.1 | 93.8% | 94% | 1_84del;195G>T;1068T>C |
| 9 | human | 10762 | NUP50 | nucleoporin 50 | XM_024452146.1 | 93.8% | 94% | 1_84del;195G>T;1068T>C |
| 10 | human | 10762 | NUP50 | nucleoporin 50 | XM_017028538.2 | 49.4% | 49.5% | 0_1ins666;318T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagtgaggaa gtcttgaaga atagagccat aaagaaagca aagcgcagaa 121 atgttggatt tgaatctgac actggaggag cctttaaagg ttttaaaggt ttggttgtac 181 cttctggagg aggacgcttt tctggatttg gtagtggcgc tggagggaag cctttggaag 241 gactgtcgaa tggaaacaac ataaccagtg cccctccctt cgccagtgca aaggcagcgg 301 cagatcccaa ggtagccttt ggttctcttg ctgcaaatgg ccctaccacc ttggttgata 361 aagtttcaaa tcccaaaact aatggggaca gtcagcagcc ctcctcctct ggccttgctt 421 ccagtaaagc ttgtgtcgga aatgcctatc acaagcagtt ggccgccttg aactgctccg 481 tgcgggattg gatagtgaag cacgtgaata caaaccccct ctgtgatctg acacctatct 541 ttaaagacta tgagaaatat ttagcaaaca ttgaacagca acacgggaac agtggcagga 601 attctgaaag tgaatctaac aaagtggcag ctgaaacaca gtctccttcc ctttttggct 661 caacaaaatt acagcaagag tcaacgtttt tgtttcatgg caacaaaact gaagatacac 721 ctgacaagaa gatggaggtg gcatctgaaa agaaaacgga cccatcatca ctaggagcga 781 caagtgcctc atttaatttc ggcaagaaag ttgatagctc tgttttgggc tcattaagct 841 ctgtccccct gactggattt tctttctccc ctggaaactc cagtttattt ggcaaagata 901 ctacccagag taaaccagtc tcttcaccat ttcccactaa accattggag ggccaagcag 961 aaggtgacag tggtgaatgc aaaggtggag atgaagaaga gaatgatgag ccacccaaag 1021 tagtagttac cGAAGTAAAA GAAGAAGACG CTTTTTACTC CAAAAAGTGT AAACTGTTTT 1081 ACAAGAAAGA CAATGAGTTT AAAGAGAAAG GCATAGGTAC TCTGCATTTA AAACCTACAG 1141 CAAATCAGAA GACACAGCTT TTGGTGCGGG CAGACACCAA TTTAGGCAAC ATATTGCTGA 1201 ACGTTCTGAT TCCACCCAAT ATGCCATGTA CGCGAACAGG GAAGAATAAC GTTCTTATCG 1261 TCTGTGTTCC AAATCCACCA ATTGACGAGA AGAATGCCAC CATGCCAGTC ACCATGTTGA 1321 TTCGGGTAAA AACCAGCGAG GATGCAGACG AGTTGCACAA AATTTTACTG GAGAAAAAGG 1381 ATGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAGGTAAAA CCAGTCTTAC AGACAACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt