Construct: ORF TRCN0000469060
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001826.1_s317c1
- Derived from:
- ccsbBroadEn_11657
- DNA Barcode:
- CAGATCAGGCACCAGCCCCTGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMCH1 (22998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469060
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_024453938.1 | 17.9% | 18% | 21_22insAAGGTT;474_2625delinsG |
| 2 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001112720.3 | 17.7% | 17.7% | 21_22insAAGGTT;475_2670del |
| 3 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001112719.3 | 17.4% | 17.4% | 21_22insAAGGTT;474_2706delinsG |
| 4 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330982.1 | 17.4% | 17.4% | 21_22insAAGGTT;474_2709delinsG |
| 5 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330674.1 | 17.2% | 17.2% | 21_22insAAGGTT;475_2748del |
| 6 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330793.1 | 16.9% | 16.9% | 21_22insAAGGTT;474_2784delinsG |
| 7 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330983.1 | 16.8% | 16.9% | 21_22insAAGGTT;474_2793delinsG |
| 8 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330786.2 | 16.8% | 16.7% | 3_4insGATTC;6_7insAAGACAAGTCAAGGTT;459_2691delinsG |
| 9 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001289124.2 | 16.6% | 16.4% | 3_4insGATTC;6_7insAAGACAAGTCAAGGTT;460_2730del |
| 10 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513655.1 | 16.6% | 16.6% | 21_22insAAGGTT;475_2835del |
| 11 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007903.1 | 16.4% | 16.4% | 21_22insAAGGTT;474_2871delinsG |
| 12 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330784.2 | 16% | 15.4% | (many diffs) |
| 13 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330789.2 | 15.3% | 14.7% | (many diffs) |
| 14 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330792.2 | 15.1% | 14.5% | (many diffs) |
| 15 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330788.2 | 14.9% | 14.3% | (many diffs) |
| 16 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001112718.4 | 14.9% | 14.3% | (many diffs) |
| 17 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001112717.4 | 14.9% | 14.3% | (many diffs) |
| 18 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007900.1 | 14.9% | 14.3% | (many diffs) |
| 19 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001289122.3 | 14.7% | 14.1% | (many diffs) |
| 20 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330791.2 | 14.7% | 14.1% | (many diffs) |
| 21 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_024453937.1 | 14.7% | 14.1% | (many diffs) |
| 22 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330790.2 | 14.5% | 13.9% | (many diffs) |
| 23 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_014988.5 | 14.5% | 13.9% | (many diffs) |
| 24 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330787.2 | 14.5% | 13.9% | (many diffs) |
| 25 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007898.1 | 14.5% | 13.9% | (many diffs) |
| 26 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513654.1 | 14.3% | 13.7% | (many diffs) |
| 27 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513653.1 | 14.1% | 13.6% | (many diffs) |
| 28 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513649.1 | 10.4% | 10.4% | 21_22insAAGGTT;474_4503delinsG |
| 29 | human | 22998 | LIMCH1 | LIM and calponin homology d... | NM_001330672.2 | 10.3% | 10.1% | 3_4insGATTC;6_7insAAGACAAGTCAAGGTT;459_4401delinsG |
| 30 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513651.2 | 10.1% | 9.9% | 3_4insGATTC;6_7insAAGACAAGTCAAGGTT;459_4488delinsG |
| 31 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007897.1 | 10% | 9.5% | (many diffs) |
| 32 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513648.1 | 10% | 9.5% | (many diffs) |
| 33 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007896.1 | 9.8% | 9.4% | (many diffs) |
| 34 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_006713996.1 | 9.8% | 9.4% | (many diffs) |
| 35 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_005248061.1 | 9.8% | 9.4% | (many diffs) |
| 36 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_005248060.1 | 9.6% | 9.2% | (many diffs) |
| 37 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513646.1 | 9.6% | 9.2% | (many diffs) |
| 38 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513645.1 | 9.6% | 9.2% | (many diffs) |
| 39 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007895.1 | 9.6% | 9.2% | (many diffs) |
| 40 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_005248058.1 | 9.6% | 9.2% | (many diffs) |
| 41 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_005248057.1 | 9.6% | 9.2% | (many diffs) |
| 42 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_017007894.1 | 9.5% | 9.1% | (many diffs) |
| 43 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513644.1 | 9.5% | 9.1% | (many diffs) |
| 44 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513643.1 | 9.5% | 9.1% | (many diffs) |
| 45 | human | 22998 | LIMCH1 | LIM and calponin homology d... | XM_011513642.1 | 9.5% | 9.1% | (many diffs) |
| 46 | mouse | 77569 | Limch1 | LIM and calponin homology d... | NM_001256122.2 | 15.3% | 16.7% | (many diffs) |
| 47 | mouse | 77569 | Limch1 | LIM and calponin homology d... | NM_001368863.1 | 15.1% | 16.3% | (many diffs) |
| 48 | mouse | 77569 | Limch1 | LIM and calponin homology d... | NM_001001980.3 | 13.1% | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttccgaaaga caagtcaagg ttaaggacac tgatgacatt gaaagtccta 121 aacgcagtat ccgagacagt ggctacatcg actgctggga ttccgagcgc agcgactccc 181 tctctcctcc tcgccacggc agagatgatt ccttcgacag cctggattcc tttggctctc 241 gctctcggca gacgccTTCA CCAGATGTAG TCCTCAGGGG AAGCAGCGAT GGGAGAGGAA 301 GCGACTCTGA ATCCGACTTG CCTCATCGGA AGCTGCCAGA TGTGAAGAAG GATGACATGT 361 CTGCACGGCG GACTTCCCAT GGTGAGCCGA AATCAGCAGT GCCTTTTAAC CAGTACCTCC 421 CGAACAAAAG CAATCAGACG GCCTACGTCC CCGCGCCTCT GAGAAAGAAG AAAGCAGAGA 481 GAGAGGAATA CCGCAAGAGC TGGAGTACCG CCACCTCCCC GCTGGGTGGG GAGAGGCCCT 541 TCAGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGATCAGG CACCAGCCCC TGCAAACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt