Construct: ORF TRCN0000469061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006105.1_s317c1
- Derived from:
- ccsbBroadEn_05814
- DNA Barcode:
- CCTGCGAGCCATAGCCCCATCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANG (283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283 | ANG | angiogenin | NM_001097577.3 | 99.7% | 99.3% | 176T>C |
| 2 | human | 283 | ANG | angiogenin | NM_001145.4 | 99.7% | 99.3% | 176T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gatgggcctg ggcgttttgt tgttggtctt cgtgctgggt ctgggtctga 121 ccccaccgac ccTGGCTCAG GATAACTCCA GGTACACACA CTTCCTGACC CAGCACTATG 181 ATGCCAAACC ACAGGGCCGG GATGACAGAT ACTGTGAAAG CATCATGAGG AGACGGGGCC 241 CGACCTCACC CTGCAAAGAC ATCAACACAT TTATTCATGG CAACAAGCGC AGCATCAAGG 301 CCATCTGTGA AAACAAGAAT GGAAACCCTC ACAGAGAAAA CCTAAGAATA AGCAAGTCTT 361 CTTTCCAGGT CACCACTTGC AAGCTACATG GAGGTTCCCC CTGGCCTCCA TGCCAGTACC 421 GAGCCACAGC GGGGTTCAGA AACGTTGTTG TTGCTTGTGA AAATGGCTTA CCTGTCCACT 481 TGGATCAGTC AATTTTCCGT CGTCCGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTGCGAG 661 CCATAGCCCC ATCATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt