Construct: ORF TRCN0000469088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016209.1_s317c1
- Derived from:
- ccsbBroadEn_15810
- DNA Barcode:
- ACGCATTCCTCCAAGCATGAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EHD4 (30844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469088
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30844 | EHD4 | EH domain containing 4 | NM_139265.4 | 99.9% | 100% | 846T>C |
| 2 | mouse | 98878 | Ehd4 | EH-domain containing 4 | NM_133838.4 | 90.8% | 97% | (many diffs) |
| 3 | mouse | 98878 | Ehd4 | EH-domain containing 4 | XM_017319352.1 | 75.6% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1689
- ORF length:
- 1623
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagctggatg gggcggcagg cgggcgggcg cgaacgcgct ggcggcgcgg 121 acgcggtgca gacggtgacg ggcgggctgc gctcgctcta cctgcgcaag gtgctgccgc 181 tggaggaggc gtaccgcttc cacgagttcc actcgcctgc gctggaggac gccgacttcg 241 agaacaagcc catgatcctg ctggtgggcc agtacagcac cggcaagacc accttcatca 301 gatacctact ggagcaggat ttcccaggca tgaggattgg tccggagccc accacagact 361 ccttcatcgc cgtgatgtat ggagagactg agggcagcac cccagggaat gctttagtcg 421 tggaccccaa aaagccgttt agaaagctca gtcgctttgg aaacgctttc ctgaatcgat 481 tcatgtgctc acagctcccc aatcaggtcc tgaagagcat cagcgtcatc gacagccccg 541 gcatcctttc tggggagaag cagcgcatca gccgaggcta tgacttctgc caggtcctgc 601 agtggtttgc cgagagggtg gacaggatca tcctgctctt tgacgctcac aagctggaca 661 tctcagatga attctcagag gccatcaagg ccttccgggg ccaggacgac aagatccgtg 721 tcgtgctgaa caaggccgac caagtggaca cgcagcagct gatgcgggtc tacggggccc 781 tcatgtggtc cctaggcaag gtcatcaaca cgcccgaggt actgcgcgtc tacattggct 841 ccttctgggc gcagcccctg cagaacacgg acaaccgccg gctcttcgag gctgaggccc 901 aggacctctt cagagacatc cagagcctcc cccagaaggc agcggtgcgc aagctcaacg 961 acctcatcaa gcgagcgagg ctggccaagg tgcatgccta catcatcagc tacctgaaga 1021 aggagatgcc aagtgtattt ggaaaggaaa acaagaagag agagcttatc agcaggctac 1081 cggaaatcta cattcagcta cagcgagaat accagatttc tgcaggggac ttccctgagg 1141 tcaaggctat gcaggaacag cttgagaact atgacttcac caaattccac tcgctgaagc 1201 ccaagctgat cgaggcagtg gacaacatgc tgagcaacaa gatctcgccc cTCATGAACC 1261 TCATCAGCCA GGAGGAGACG AGCACGCCCA CGCAGCTGGT GCAGGGCGGC GCCTTCGATG 1321 GCACCACCGA GGGCCCCTTC AACCAGGGCT ACGGGGAGGG TGCCAAGGAG GGCGCCGACG 1381 AGGAGGAGTG GGTCGTGGCC AAAGACAAGC CCGTCTACGA CGAGCTCTTC TACACTCTGT 1441 CGCCCATCAA TGGCAAGATA TCAGGTGTCA ACGCCAAGAA GGAGATGGTG ACCTCCAAGC 1501 TGCCCAACAG CGTCCTGGGC AAGATCTGGA AGCTGGCCGA CTGCGACTGC GACGGCATGC 1561 TTGATGAGGA GGAGTTCGCG CTGGCCAAGC ACCTCATCAA GATCAAGCTC GACGGCTACG 1621 AGCTGCCCAG CAGCCTGCCC CCCCACCTCG TGCCCCCCTC GCACAGGAAG TCCCTGCCCA 1681 AGGCCGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1741 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1801 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACGCAT TCCTCCAAGC ATGAGTTCAC 1861 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt