Construct: ORF TRCN0000469098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014750.1_s317c1
- Derived from:
- ccsbBroadEn_07712
- DNA Barcode:
- TCCGGTAGCTCCCGCCCTCTCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SF3A3 (10946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10946 | SF3A3 | splicing factor 3a subunit 3 | NM_006802.4 | 99.8% | 99.8% | 399A>G;1495G>C |
| 2 | human | 10946 | SF3A3 | splicing factor 3a subunit 3 | NM_001320830.2 | 89.2% | 89.2% | 144_145ins159;240A>G;1336G>C |
| 3 | mouse | 75062 | Sf3a3 | splicing factor 3a, subunit 3 | NM_029157.3 | 91.1% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacaatactg gagcagcagc ggcgctatca tgaggagaag gaacggctca 121 tggacgtcat ggctaaagag atgctcacca agaagtccac gctccgggac cagatcaatt 181 ctgatcaccg cactcgggcc atgcaagata ggtatatgga ggtcagtggg aacctgaggg 241 atttgtatga tgataaggat ggattacgaa aggaggagct caatgccatt tcaggaccca 301 atgagtttgc tgaattctat aatagactca agcaaataaa ggaattccac cggaagcacc 361 caaatgagat ctgtgtgcca atgtcagtgg aatttgagga actcctgaag gctcgagaga 421 atccaagtga agaggcacaa aacttggtgg agttcacaga tgaggaggga tatggtcgtt 481 atctcgatct ccatgactgt tacctcaagt acattaacct gaaggcatct gagaagctgg 541 attatatcac atacctgtcc atctttgacc aattatttga cattcctaaa gaaaggaaga 601 atgcagagta taagagatac ctagagatgc tgcttgagta ccttcaggat tacacagata 661 gagtgaagcc tctccaagat cagaatgaac tttttgggaa gattcaggct gagtttgaga 721 agaaatggga gaatgggacc tttcctggat ggccgaaaga gacaagcagt gccctgaccc 781 atgctggagc ccatcttgac ctctctgcat tctcctcctg ggaggagttg gcttctctgg 841 gtttggacag attgaaatct gctctcttag ctttaggctt gaaatgtggc gggaccctag 901 aagagcgagc ccagagacta ttcagtacca aaggaaagtc cctggagtca cttgatacct 961 ctttgtttgc caaaaatccc aagtcaaagg gcaccaagcg agacactgaa aggaacaaag 1021 acattgcttt tctagaagcc cagatctatg aatatgtaga gattctcggg gaacagcgac 1081 atctcacTCA TGAAAATGTA CAGCGCAAGC AAGCCAGGAC AGGAGAAGAG CGAGAAGAAG 1141 AGGAAGAAGA GCAGATCAGT GAGAGTGAGA GTGAAGATGA AGAGAACGAG ATCATTTACA 1201 ACCCCAAAAA CCTGCCACTT GGCTGGGATG GCAAACCTAT TCCCTACTGG CTGTATAAGC 1261 TTCATGGCCT AAATATCAAC TACAACTGTG AGATTTGTGG AAACTACACC TACCGAGGGC 1321 CCAAAGCCTT CCAGCGACAC TTTGCTGAAT GGCGTCATGC TCATGGCATG AGGTGTTTGG 1381 GCATCCCAAA CACTGCTCAC TTTGCTAATG TGACACAGAT TGAAGATGCT GTCTCCTTGT 1441 GGGCCAAACT GAAATTGCAG AAGGCTTCAG AACGATGGCA GCCTGACACT GAGGAAGAAT 1501 ATGAAGACTC AAGTGGGAAT GTTGTGAATA AGAAGACATA CGAGGATCTG AAAAGACAAC 1561 GACTGCTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCGGT AGCTCCCGCC CTCTCACCAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt