Construct: ORF TRCN0000469099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014889.1_s317c1
- Derived from:
- ccsbBroadEn_01710
- DNA Barcode:
- CTCCTAACGACTTCTATATGAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAF2 (7186)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7186 | TRAF2 | TNF receptor associated fac... | NM_021138.4 | 100% | 100% | |
| 2 | human | 7186 | TRAF2 | TNF receptor associated fac... | XM_011518974.2 | 100% | 100% | |
| 3 | human | 7186 | TRAF2 | TNF receptor associated fac... | XM_011518976.3 | 100% | 100% | |
| 4 | human | 7186 | TRAF2 | TNF receptor associated fac... | XM_011518977.2 | 100% | 100% | |
| 5 | mouse | 22030 | Traf2 | TNF receptor-associated fac... | NM_009422.3 | 83.9% | 86.8% | (many diffs) |
| 6 | mouse | 22030 | Traf2 | TNF receptor-associated fac... | NM_001290413.1 | 82.7% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagctagc gtgacccccc ctggctccct ggagttgcta cagcccggct 121 tctccaagac cctcctgggg accaagctgg aagccaagta cctgtgctcc gcctgcagaa 181 acgtcctccg caggcccttc caggcgcagt gtggccaccg gtactgctcc ttctgcctgg 241 ccagcatcct cagctctggg cctcagaact gtgctgcctg tgttcacgag ggcatatatg 301 aagaaggcat ttctatttta gaaagcagtt cggccttccc agataatgct gcccgcaggg 361 aggtggagag cctgccggcc gtctgtccca gtgatggatg cacctggaag gggaccctga 421 aagaatacga gagctgccac gaaggccgct gcccgctcat gctgaccgaa tgtcccgcgt 481 gcaaaggcct ggtccgcctt ggtgaaaagg agcgccacct ggagcacgag tgcccggaga 541 gaagcctgag ctgccggcat tgccgggcac cctgctgcgg agcagacgtg aaggcgcacc 601 acgaggtctg ccccaagttc cccttaactt gtgacggctg cggcaagaag aagatccccc 661 gggagaagtt tcaggaccac gtcaagactt gtggcaagtg tcgagtccct tgcagattcc 721 acgccatcgg ctgcctcgag acggtagagg gtgagaaaca gcaggagcac gaggtgcagt 781 ggctgcggga gcacctggcc atgctactga gctcggtgct ggaggcaaag cccctcttgg 841 gagaccagag ccacgcgggg tcagagctcc tgcagaggtg cgagagcctg gagaagaaga 901 cggccacttt tgagaacatt gtctgcgtcc tgaaccggga ggtggagagg gtggccatga 961 ctgccgaggc ctgcagccgg cagcaccggc tggaccaaga caagattgaa gccctgagta 1021 gcaaggtgca gcagctggag aggagcattg gcctcaagga cctggcgatg gctgacttgg 1081 agcagaaggt cttggagatg gaggcatcca ccTACGATGG GGTCTTCATC TGGAAGATCT 1141 CAGACTTCGC CAGGAAGCGC CAGGAAGCTG TGGCTGGCCG CATACCCGCC ATCTTCTCCC 1201 CAGCCTTCTA CACCAGCAGG TACGGCTACA AGATGTGTCT GCGTATCTAC CTGAACGGCG 1261 ACGGCACCGG GCGAGGAACA CACCTGTCCC TCTTCTTTGT GGTGATGAAG GGCCCGAATG 1321 ACGCCCTGCT GCGGTGGCCC TTCAACCAGA AGGTGACCTT AATGCTGCTC GACCAGAATA 1381 ACCGGGAGCA CGTGATTGAC GCCTTCAGGC CCGACGTGAC TTCATCCTCT TTTCAGAGGC 1441 CAGTCAACGA CATGAACATC GCAAGCGGCT GCCCCCTCTT CTGCCCCGTC TCCAAGATGG 1501 AGGCAAAGAA TTCCTACGTG CGGGACGATG CCATCTTCAT CAAGGCCATT GTGGACCTGA 1561 CAGGGCTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCCTA ACGACTTCTA TATGAGATAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt