Construct: ORF TRCN0000469128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005079.1_s317c1
- Derived from:
- ccsbBroadEn_13163
- DNA Barcode:
- CTCGTGACATGCTAATCGGTAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPRA1 (131601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001142646.4 | 77.7% | 72.6% | (many diffs) |
| 2 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353006.2 | 68% | 52% | 496_566del;789_1053del |
| 3 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001136053.4 | 64% | 56.2% | 610_670del;779_1119del |
| 4 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353001.2 | 64% | 56.2% | 610_670del;779_1119del |
| 5 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353002.2 | 64% | 56.2% | 610_670del;779_1119del |
| 6 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353003.2 | 64% | 56.2% | 610_670del;779_1119del |
| 7 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353004.2 | 64% | 56.2% | 610_670del;779_1119del |
| 8 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353005.2 | 64% | 56.2% | 610_670del;779_1119del |
| 9 | human | 131601 | TPRA1 | transmembrane protein adipo... | XM_006713496.3 | 64% | 56.2% | 610_670del;779_1119del |
| 10 | human | 131601 | TPRA1 | transmembrane protein adipo... | NM_001353007.2 | 60.3% | 51.7% | (many diffs) |
| 11 | human | 131601 | TPRA1 | transmembrane protein adipo... | XR_001740017.1 | 43.2% | 1_141del;859_1658del | |
| 12 | human | 131601 | TPRA1 | transmembrane protein adipo... | XR_924102.2 | 39.9% | 1_277del;995_1794del | |
| 13 | human | 131601 | TPRA1 | transmembrane protein adipo... | XR_001740016.2 | 34.6% | 1_552del;1270_2069del | |
| 14 | human | 131601 | TPRA1 | transmembrane protein adipo... | NR_073377.3 | 19.4% | 1_341del;1059_3691del | |
| 15 | human | 131601 | TPRA1 | transmembrane protein adipo... | NR_148226.2 | 18.7% | (many diffs) | |
| 16 | mouse | 24100 | Tpra1 | transmembrane protein, adip... | NM_011906.2 | 55.2% | 50.6% | (many diffs) |
| 17 | mouse | 24100 | Tpra1 | transmembrane protein, adip... | XM_006506061.2 | 55.2% | 50.6% | (many diffs) |
| 18 | mouse | 24100 | Tpra1 | transmembrane protein, adip... | XM_006506062.2 | 55.2% | 50.6% | (many diffs) |
| 19 | mouse | 24100 | Tpra1 | transmembrane protein, adip... | XM_006506063.2 | 55.2% | 50.6% | (many diffs) |
| 20 | mouse | 24100 | Tpra1 | transmembrane protein, adip... | XM_006506064.2 | 32.5% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caccctggag gaggtgactt gggccaatgg gagcacagcg ctacccccac 121 ccctggcacc aaacatcagt gtgcctcatc gctgcctgct gctgctctac gaagacattg 181 gcacctccag ggtccggtac tgggacctct tgctgctcat ccccaatgtg ctcttcctca 241 tcttcctgct ctggaagctt ccatctgctc gggcgaagat ccgcatcacc tccagcccca 301 tttttatcac cttctacatc ctggtgtttg tggtggcgct ggtgggcatt gcccgggccg 361 tggtatccat gacggtgagc acctcgaacg ctgcaactgt tgctgataag atcctgtggg 421 agaTCACCCG CTTCTTCCTG CTGGCCATCG AGCTGAGTGT GATCATCCTG GGCCTGGCCT 481 TTGGCCACCT GGAGAGTAAG TCCAGCATCA AGCGGGTGCT GGCCATCACC ACAGTGCTGT 541 CCCTGGCCTA CTCTGTCACC CAGGGGACCC TGGAGATCCT GTACCCTGAT GCCCATCTCT 601 CAGCTGAGGA CTTTAATATC TATGGCCATG GGGGCCGCCA GTTCTGGCTG GTCAGCTCCT 661 GCTTCTTCTT CCTGCTCGGA GGAGCTTCTA CGTGTATGCG GGCATCCTGG CACTGCTCAA 721 CCTACTGCAG GGGCTGGGGA GTGTGCTGCT GTGCTTCGAC ATCATCGAGG GGCTCTGCTG 781 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGACT CGTGACATGC TAATCGGTAG ACACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt