Construct: ORF TRCN0000469138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002268.1_s317c1
- Derived from:
- ccsbBroadEn_12928
- DNA Barcode:
- CGTAGCACTGTTAGGAGCTACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE3B (89910)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270449.1 | 100% | 100% | |
| 2 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270450.1 | 100% | 100% | |
| 3 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270451.1 | 100% | 100% | |
| 4 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_017020196.1 | 100% | 100% | |
| 5 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_011538961.1 | 22.4% | 19.9% | (many diffs) |
| 6 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_006719682.2 | 22.1% | 19.7% | (many diffs) |
| 7 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_006719681.3 | 22.1% | 19.7% | (many diffs) |
| 8 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_130466.4 | 21.6% | 19.3% | (many diffs) |
| 9 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_183415.3 | 21.6% | 19.3% | (many diffs) |
| 10 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_005253987.2 | 21.6% | 19.3% | (many diffs) |
| 11 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_011538959.2 | 21.6% | 19.3% | (many diffs) |
| 12 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XR_429119.4 | 20% | (many diffs) | |
| 13 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XR_429118.3 | 19.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt caccctgtct cagacctcga gagcatggtt catcgataga gcccgtcagg 121 cacgagaaga aaggcttgtg cagaaggaac gggagcgggc agctgttgtg atccaggccc 181 atgtccggag ttttctctgt cggagtcgac tgcagagaga tatcaggaga gagattgatg 241 acttttttaa agcagatgac cctgagtcca ctaaaagaag tgcactttgt attttcaaga 301 ttgccaggaa actgctgttc ctattcagaa tcaaagagga taatgagaga tttgagaagt 361 tgtgtcgcag catccTGAGC AGCATGGATG CTGAGAATGA GCCTAAGGTG TGGTATGTGT 421 CCCTGGCTTG TTCTAAGGAC CTCACCCTCC TTTGGATTCA ACAGATCAAG AACATTTTGT 481 GGTACTGCTG TGATTTTCTC AAGCAGCTCA AGCCTGAAAT CCTGCAGGAC TCCCGACTCA 541 TCACCCTGTA CCTCACGATG CTTGTCACCT TCACAGACAC TTCAACGTGG AAAATTCTTC 601 GGGGAAAAGG TGAAAGTCTT CGACCAGCGA TGAACCACAT TTGTGCAAAT ATAATGGGAC 661 ATCTCAACCA GCATGGATTT TATTCTGTGC TGCAGTGCTG TGATGGGCTG TTTCCTGATT 721 TGGTTTCATA TGCTCCTCAC AACAACCCTG TGAGGTGGTC CGTTGGCAGA AGCTGGTATG 781 ACTGGCAGTT GTCTCGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGTAGCA CTGTTAGGAG 961 CTACTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt