Construct: ORF TRCN0000469140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007921.1_s317c1
- Derived from:
- ccsbBroadEn_08856
- DNA Barcode:
- GGTCTTACCACGACGTACAGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAI2 (64446)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64446 | DNAI2 | dynein axonemal intermediat... | NM_001172810.3 | 99.9% | 99.8% | 1636G>A |
| 2 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450882.1 | 99.9% | 99.8% | 1636G>A |
| 3 | human | 64446 | DNAI2 | dynein axonemal intermediat... | NM_023036.6 | 97.9% | 97.8% | 1346_1381del;1672G>A |
| 4 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450880.1 | 93% | 92.9% | 1636G>A;1687_1818del |
| 5 | human | 64446 | DNAI2 | dynein axonemal intermediat... | NM_001353167.2 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 6 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_011525125.2 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 7 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450874.1 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 8 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450875.1 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 9 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450876.1 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 10 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450877.1 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 11 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450878.1 | 91.3% | 91.2% | 1346_1381del;1672G>A;1723_1854del |
| 12 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450879.1 | 89.8% | 87.8% | (many diffs) |
| 13 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450883.1 | 88% | 82.7% | 1346_1381del;1567_1568ins155;1635_1636ins25 |
| 14 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450884.1 | 88% | 82.7% | 1346_1381del;1567_1568ins155;1635_1636ins25 |
| 15 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450881.1 | 85.4% | 85.3% | (many diffs) |
| 16 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450885.1 | 69.2% | 67.4% | (many diffs) |
| 17 | human | 64446 | DNAI2 | dynein axonemal intermediat... | XM_024450886.1 | 69.2% | 67.4% | (many diffs) |
| 18 | human | 64446 | DNAI2 | dynein axonemal intermediat... | NR_148379.2 | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gattgtgtac gtgtacgtca agaagcgcag cgagttcggg aagcagtgca 121 atttctcgga ccgccaggcc gagctgaaca tcgacatcat gcccaaccct gagctggccg 181 agcagttcgt ggagcggaac ccagtggaca cgggcatcca gtgctcgatc agcatgtcgg 241 aacacgaggc caactcagag cggtttgaga tggagacccg gggagttaac catgtcgagg 301 ggggctggcc caaggacgtg aaccccctgg agctggagca gaccatccgt ttccggaaga 361 aagtggagaa agatgagaac tacgttaacg ccatcatgca gctcggctct atcatggagc 421 actgcatcaa gcagaacaat gccattgaca tctatgaaga gtatttcaat gacgaggagg 481 ccatggaagt gatggaggag gacccttcag ctaaaaccat caatgtgttc agggaccccc 541 aggaaatcaa gagggctgcc acacacctct cctggcaccc cgatggcaac aggaagttgg 601 cagtggcata ctcctgcttg gattttcagc gggcacctgt gggcatgagc agcgattcat 661 acatctggga cctggaaaac cccaacaagc ctgaacttgc tctgaagcca tcgtctccac 721 tcgtgacgtt ggagttcaac cccaaagatt cccacgtact cctgggtggc tgctacaatg 781 gacagatagc ctgctgggac acccgaaagg gcagcctggt ggcggagcta tccaccattg 841 agtccagcca ccgagaccct gtgtatggca ccatctggct gcagtcgaag acgggcaccg 901 agtgcttctc agcttccacg gatgggcagg tcatgtggtg ggacatccga aagatgagcg 961 agcccactga agttgtgatc ttggacatca ccaagaagga acagttggaa aatgccttgg 1021 gggccatctc cctggagttc gaatctactt tgcccaccaa gttcatggtg gggaccgagc 1081 agggcatcgt catctcctgc aaccgcaagg ccaagacgtc agctgaaaag attgtgtgca 1141 ccttcccggg ccatcatggc cccatctacg ccctccagag aaaccccttc tacccgaaga 1201 acttcctgac ggttggcgac tggacagccc gcatttggtc tgaagacagc cgggaatcgt 1261 ccatcatgtg gaccaagtac cacatggctt acctcactga tgctgcctgg agccccgtga 1321 ggccgaccgt tttctttacc accaggatgg acggaaccct ggatatctgg gacttcatgt 1381 tcgagcagtg cgatcccacc ctcagcttga aggacaatgg gtgtctcatc gccTGCGGCT 1441 CCCAGCTGGG GACAACCACC CTGCTGGAGG TCTCGCCTGG GCTCTCTACC CTCCAGAGGA 1501 ATGAGAAGAA CGTAGCCTCT TCCATGTTTG AGCGTGAGAC CCGGCGAGAG AAGATCCTGG 1561 AGGCCAGGCA CCGGGAGATG CGGCTGAAGG AGAAGGGTAA GGCGGAGGGC AGGGATGAGG 1621 AGCAGACCGA TGAGGAGCTG GCCGTAGACC TGGAGGCGCT GGTCAGCAAG GCCGAGGAGG 1681 AGTTCTTCGA CATCATCTTC ACAGAGCTGA AGAAGAAGGA GGCAGACGCC ATAAAGCTGA 1741 CGCCAGTGCC TCAGCAACCA AGTCCAGAAG AAGACCAGGT GGTGGAGGAG GGAGAGGAAG 1801 CAGCGGGGGA AGAAGGGGAT GAAGAAGTGG AAGAAGACTT AGCCTACCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA GGTCTTACCA CGACGTACAG TCGAACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt