Construct: ORF TRCN0000469152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003677.1_s317c1
- Derived from:
- ccsbBroadEn_14453
- DNA Barcode:
- CGCTACCGTTGCTGATTCCTCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ELMOD2 (255520)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469152
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255520 | ELMOD2 | ELMO domain containing 2 | NM_153702.4 | 99.8% | 2.7% | 12delT |
| 2 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XM_005262886.2 | 99.8% | 2.7% | 12delT |
| 3 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XM_011531819.2 | 99.8% | 2.7% | 12delT |
| 4 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XM_005262885.3 | 95.9% | 2.6% | 1_36del;48delT |
| 5 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XM_011531818.2 | 86.3% | 2.7% | (many diffs) |
| 6 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XR_938716.2 | 61.6% | (many diffs) | |
| 7 | human | 255520 | ELMOD2 | ELMO domain containing 2 | XR_001741193.1 | 12.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 117
- ORF length:
- 51
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tatttcttgt gggagttctt ctatgggcac ttttttcgat tttggatgaa 121 atggctatta cgacagatga ctgggaagtg tgaattgcag cgaatatttg atacctatgt 181 aggtgcacaa aggacacaca ggatagaaaa ttccttgaca tactccaaga ataaggtttt 241 acagaaggcg acacatgttg ttcagagtga agtggacaaa tatgtagatg atattatgaa 301 ggaaaagaat attaaccctg agaaggatgc cagttttaaa atatgcatga agatgtgctt 361 actgcagata actggttata aacagctgta tttggatgta gaaagtgtga ggaaaaggcc 421 atatgattct gataacctac agcatgaaga gctactcatg aagctttgga atcttctaat 481 gcccacgaag aagttaaacg ctagaatctc caagcagtgg gctgaaattg gttttcaggg 541 tgatgatccC AAGACAGACT TCAGAGGCAT GGGCATACTT GGGTTAATCA ATCTTGTGTA 601 TTTCAGTGAA AATTACACTA GTGAAGCTCA TCAGATTCTT TCCCGTTCAA ATCATCCAAA 661 ATTAGGGTAT TCTTATGCAA TAGTTGGAAT CAATCTTACA GAGATGGCTT ATAGCTTACT 721 GAAGAGTGAA GCTTTGAAGT TTCATCTCTA TAACCTTGTT CCTGGTATAC CAACAATGGA 781 ACACTTTCAT CAGTTTTACT GTTATCTTGT CTATGAATTT GACAAGTTTT GGTTTGAAGA 841 AGAACCAGAA AGCATTATGT ATTTCAATTT GTATAGAGAG AAGTTTCATG AAAAGATTAA 901 AGGACTTTTA CTGGATTGTA ATGTAGCACT TACTTTAAAA GTATACCCAA CTTTCTTGTA 961 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 1021 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 1081 AAAGGACGAC GCTACCGTTG CTGATTCCTC CAAACGCGTT AAGTCgacaa tcaacctctg 1141 gattacaaaa tttgtgaaag att