Construct: ORF TRCN0000469160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011090.1_s317c1
- Derived from:
- ccsbBroadEn_13684
- DNA Barcode:
- GTTCCGTCCTGGCTCCTCCGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC439933 (439933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 439933 | LOC439933 | uncharacterized LOC439933 | NR_122080.1 | 26.7% | 1_15del;58T>C;370_1318del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaggctaaa ctttttcttc ccacatgctc acttcctcgc ttgttcgcta 121 gctgctgcaT CTGGCCCCTC AAGGGTAAAG TCAAGGGGAA AAGGAGATTG AGAGGAAGGA 181 CAGTCTGTGA ATTGGGTCTC ACCGTGTTTC CCAGGCTGAA GTACAGTGTT GTGATATCAG 241 TTCATTGCAA CTTCTGTCTT CTGAGCTCAA GCAATCCTCT TGCCTCAGCC TCTCAAGTAG 301 CTGGGACTGA CTACAGGCAT GAACCACCAC ACCTGGCTAA TTTTTTTTGT ATTTTTGTCG 361 AAAAGACAGT TTCGCCATGT TGTACAGAAC TACTTCTCAA ATCACTATTC ACATCCTGGT 421 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAGTTCC GTCCTGGCTC CTCCGTCGGA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt