Construct: ORF TRCN0000469161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012384.1_s317c1
- Derived from:
- ccsbBroadEn_07834
- DNA Barcode:
- CGATCTATAGTCTGGTGGCTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GGA2 (23062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23062 | GGA2 | golgi associated, gamma ada... | NM_015044.4 | 99.8% | 99.8% | 90C>G;1270G>C |
| 2 | human | 23062 | GGA2 | golgi associated, gamma ada... | XM_024450200.1 | 93.9% | 93.8% | 0_1ins111;1159G>C |
| 3 | human | 23062 | GGA2 | golgi associated, gamma ada... | XM_017023075.1 | 85.7% | 85.6% | 0_1ins261;1009G>C |
| 4 | mouse | 74105 | Gga2 | golgi associated, gamma ada... | NM_028758.2 | 82.2% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1905
- ORF length:
- 1839
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgaccgcg gtggcggcgg ctgtggcggg aaccgagtcg gcccagggtc 121 ccccgggccc ggcagcgtcg ctggagctgt ggctgaacaa agccacagac ccaagcatgt 181 cggaacagga ttggtcagct atccagaatt tctgtgagca ggtgaacact gaccccaatg 241 gccccacaca tgcgccctgg ctactggccc acaagatcca gtctccgcaa gagaaggaag 301 ctctttatgc cttaacggtg ctggagatgt gcatgaacca ctgtggggag aagttccaca 361 gcgaggtggc caaatttcgt ttcctgaacg aactgatcaa agtgttgtcc ccaaagtacc 421 tggggtcctg ggccacagga aaagttaaag gaagagtcat tgaaatactc ttcagttgga 481 cagtctggtt tccggaagac atcaagattc gagacgctta tcagatgctg aagaaacaag 541 gaattataaa acaagaccct aaactaccag tggataaaat cttaccccca ccatctccct 601 ggcccaagag ctccatcttt gatgctgatg aagaaaagtc caagcttctg acaaggcttc 661 taaagagcaa ccaccccgag gaccttcagg ctgcaaaccg gttaatcaag aatttggtca 721 aggaggaaca agaaaaatcg gagaaggtgt ccaagagggt cagtgcggtg gaggaagtgc 781 gaagccatgt gaaggtgctg caggagatgc tgagcatgta ccgcaggcca gggcaggccc 841 cgcccgacca ggaggccctg caggtcgtgt atgagaggtg tgaaaagctg cggcccacgc 901 tgttccggtt ggcgagtgac accactgatg acgatgatgc actcgcggaa attctccagg 961 caaatgacct cctcacccaa ggagttctgc tgtacaaaca ggtgatggag ggccgggtca 1021 cctttggaaa cagagtgacc agctcattgg gagacatccc tgtctccaga gtctttcaga 1081 atccagcagg ctgcatgaag acctgccccc tgattgactt ggaggtggac aatggacctg 1141 cgcagatggg gactgtggtg ccatctttgc ttcatcagga cctggcagcc ttgggaatca 1201 gtgatgctcc tgttacaggc atggtttctg gtcagaattg ctgtgaggaa aagaggaatc 1261 cctcctccag cacgctgcca ggcggtggtg ttcagaaccc ttctgcagac aggaatttgc 1321 tggacctcct ctcaccacag ccagctccgt gccctctgaa ttatgtttcg cagaaaagtg 1381 tccccaagga agtgccacca ggtactaagt cctctccagg ttggtcctgg gaggctggcc 1441 cgttggctcc ttccccatct tcacagaata cacctctggc tcaagtgttt gtccctttgg 1501 agtctgttaa gcccagcagc ctgccgcctc tcattgtgta tgaccggaat ggattcagaa 1561 tTCTGCTCCA CTTCTCCCAG ACGGGAGCCC CTGGGCACCC AGAGGTACAG GTGCTGCTCT 1621 TGACCATGAT GAGCACGGCT CCCCAGCCTG TCTGGGATAT CATGTTTCAA GTGGCTGTGC 1681 CAAAGTCAAT GAGAGTGAAG CTGCAGCCGG CATCCAGCTC CAAGCTTCCT GCATTCAGTC 1741 CTTTGATGCC TCCAGCTGTG ATATCTCAGA TGCTGCTGCT TGACAATCCA CACAAAGAAC 1801 CTATCCGCTT ACGGTACAAG CTGACATTCA ACCAAGGTGG ACAGCCTTTC AGCGAAGTAG 1861 GAGAAGTGAA AGACTTCCCA GACCTGGCTG TCTTGGGCGC AGCCTACCCA ACTTTCTTGT 1921 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1981 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2041 GAAAGGACGA CGATCTATAG TCTGGTGGCT GGACACGCGT TAAGTCgaca atcaacctct 2101 ggattacaaa atttgtgaaa gatt