Construct: ORF TRCN0000469170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012142.1_s317c1
- Derived from:
- ccsbBroadEn_12682
- DNA Barcode:
- GTTCGGTCACCCGGCATAATACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPG11 (80208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XR_001751402.1 | 57.5% | (many diffs) | |
| 2 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XM_006720701.3 | 47.5% | 47.3% | (many diffs) |
| 3 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XM_017022635.2 | 47.1% | 46.9% | (many diffs) |
| 4 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XR_931917.2 | 41.2% | (many diffs) | |
| 5 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | NM_001160227.2 | 32.3% | 32.2% | (many diffs) |
| 6 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XM_006720700.1 | 31.4% | 31.3% | (many diffs) |
| 7 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | XM_017022634.1 | 31.3% | 31.2% | (many diffs) |
| 8 | human | 80208 | SPG11 | SPG11 vesicle trafficking a... | NM_025137.4 | 30.8% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2340
- ORF length:
- 2274
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcgggttcta ccgatgctgt tggtgccagt ccccgccgag gcgatggggc 121 agctcggctc ccgggcgcag ctgcgcacac agccggaggc tctggggagc ctgacggctg 181 cgggcagcct ccaagtgctt tctttgacgc ctggcagccg gggcgggggt cgctgctgcc 241 tggagggccc cttctggcac tttctatggg aggattctcg taacagcagc acaccaactg 301 aaaagcccaa actgctcgct cttggtgaaa attatgaact gcttatctat gaatttaatt 361 tgaaagatgg aagatgtgat gcaaccattt tgtatagctg tagtagggag gcattgcaaa 421 agctcattga cgatcaagat atcagtattt ccttattgtc tttgagaatc ctgtcatttc 481 acaataacac atcattactg ttcatcaaca aatgtgtcat cctacatatt atatttcctg 541 aaagagatgc tgcaattaga gtactcaact gtttcacact tcccttgcct gcacaggcag 601 tggacatgat tattgacacg cagctctgca gaggaattct ttttgttttg agtagtttag 661 gctggatcta catttttgat gttgtggatg gtacatatgt agctcatgtg gatttagcac 721 ttcacaaaga agacatgtgt aatgagcagc aacaggagcc agccaagatt tcttcattta 781 cttcactgaa agtttctcaa gacctcgatg ttgcagtgat tgtcagctcc tccaactccg 841 cagttgctct taacttaaat ttgtatttca ggcaacaccc aggacaccta ctgtgtgaaa 901 gaatactaga agatcttcct attcaaggac ctaagggcgt agatgaagat gatcctgtta 961 actctgccta caacatgaaa ctggcaaagt tttccttcca aattgatagg tcttggaaag 1021 cccagctatc atcattggat gaaacaataa agaactccaa actggaggtt tcctgttgtg 1081 ctccatggtt ccaggatatt ttgcatttgg agtcacctga atctggtaac cacagtacaa 1141 gtgtgcagag ctgggccttc attccacagg acataatgca tgggcaatat aatgttctac 1201 agaaagatca tgccaagacc agtgatccag gaagatcatg gaaaataatg cacatcagtg 1261 aacaagagga acccatagag cttaaatgtg tgtctgtgac aggattcact gcactgttta 1321 cttgggaagt ggaaaggatg ggctatacca ttaccctctg ggatttggag acccagggca 1381 tgcagtgttt ttcccttggc acaaagtgta ttcctgtaga cagtagtgga gaccagcagc 1441 tgtgctttgt tttgacagag aatggactct ctctgatttt gtttggtttg actcaagaag 1501 agtttttaaa cagactcatg atccatggaa gtgccagcac tgtggacact ctttgtcatc 1561 tcaatggctg gggaaggtgc tcaattccca tacatgcact agaggccggg atagaaaatc 1621 gtcagctgga cacagtaaat ttctttttga agagcaagga aaatcttttt aatccatcct 1681 caaaatcttc tgtatctgat cagtttgatc acttgtcatc ccatttatat ttaagaaatg 1741 tggaagagct gataccagca ttggatttac tttgctcggc aattagagaa agttattctg 1801 aaccccaaag caaacacttt tcagaacaat tgcttaatct tacactgtct ttccttaaca 1861 accaaataaa ggagcttttc attcacactg aagaactaga tgaacatctg caaaaaggag 1921 tgaacatttt gactagctac attaatgaac ttcgaacctt catgataaag tttccttgga 1981 agctaacaga tgctatagat gaatatgatg tacatgaaaa tgtcccCAAA GTAAAGGAGA 2041 GCAATATATG GAAGAAACTC AGCTTTGAGG AAGTTATTGC CAGCGCCATT TTAAACAACA 2101 AAATACCAGA GGCACAGACT TTCTTCAGGA TTGATAGTCA TTCTGCTCAA AAACTTGAGG 2161 AGCTTATTGG CATAGGCCTA AATTTGGTCT TTGACAATTT AAAAAAGAAC AATATAAAGG 2221 AAGCCTCTGA ACTTTTGAAG AATATGGGGT TTGATGTAAA AGGCCAATTG CTCAAGATCT 2281 GCTTCTATAC AACTAATAAA AATATACGTG ACTTTTTGGT AGGTAAAGGT GAGACTACAT 2341 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2401 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2461 TTTATATATC TTGTGGAAAG GACGAGTTCG GTCACCCGGC ATAATACCTA CGCGTTAAGT 2521 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt