Construct: ORF TRCN0000469174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005099.1_s317c1
- Derived from:
- ccsbBroadEn_00051
- DNA Barcode:
- TAAAGGAAATACTACTTACATTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH3B1 (221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001030010.3 | 100% | 100% | |
| 2 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_000694.4 | 92% | 92% | 162_272del |
| 3 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001161473.3 | 92% | 92% | 162_272del |
| 4 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001031615.2 | 77.5% | 72.8% | (many diffs) |
| 5 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001354345.1 | 77.5% | 72.8% | (many diffs) |
| 6 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290058.2 | 67% | 67% | 162_272del;476_477ins351 |
| 7 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290059.2 | 63.1% | 63.1% | 0_1ins477 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cccccttggg gacacgctgc ggcgactgcg ggaggccttc cacgcggggc 121 gcacgcggcc agctgagttc cgggctgcgc agctccaagg cctgggccgc ttcctgcaag 181 aaaacaagca gcttctgcac gacgcactgg cccaggacct gcacaaggcc acgcagctgg 241 actccgcctt catccggaag gagccctttg gcctggtcct catcattgcg ccctggaact 301 atccgctgaa cctgacgctg gtgcccctcg tgggagccct cgctgcaggg aactgtgtgg 361 tgctgaagcc atcggagatt agcaagaacg tcgagaagat cctggccgag gtgctgcccc 421 aatacgtgga ccagagctgc tttgctgtgg tgctgggcgg gccccaggag acggggcagc 481 tgctagagca caggttcgac tacatcttct tcacagggag ccctcgtgtg ggcaagattg 541 ttatgactgc tgccgccaag cacctgacac ctgtcaccct ggagctgggg ggcaagaacc 601 cttgctacgt ggacgacaac tgcgaccccc agaccgtggc caaccgcgtg gcctggttcc 661 gctacttcaa cgccggccag acctgcgtgg cccccgacta cgtcctatgc agccctgaga 721 tgcaggagag gctgctgcct gccctgcaga gcaccatcac ccgtttctat ggcgacgacc 781 cccagagctc cccaaacctg ggccgcatca tcaaccagaa acagttccag cggctgcggg 841 cattgctggg ctgcggccgt gtggccattg ggggccagag cgatgagagc gatcgctaca 901 tcgcccccac ggtgctggtg gatgtgcagg agatggagcc tgtgatgcag gaggagatct 961 tcgggcccat cctgcccatc gtgaacgtgc agagcttgga cgaggccatc gagttcaTCA 1021 ACCGGCGGGA GAAGCCCCTG GCCCTGTACG CCTTCTCCAA CAGCAGCCAG GTGGTCAAGC 1081 GGGTGCTGAC CCAGACCAGC AGCGGGGGCT TCTGTGGGAA CGACGGCTTC ATGCACATGA 1141 CCCTGGCCAG CCTGCCTTTT GGAGGAGTGG GTGCCAGTGG GATGGGCCGG TACCATGGCA 1201 AGTTCTCCTT CGACACCTTC TCCCACCATC GCGCCTGCCT CCTGCGCAGC CCGGGGATGG 1261 AGAAGCTCAA CGCCCTCCGC TACCCGCCGC AATCGCCGCG CCGCCTGAGG ATGCTGCTGG 1321 TGGCCATGGA GGCCCAAGGC TGCAGCTGCA CACTGCTCTG CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGATAAAGG AAATACTACT TACATTATAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt