Transcript: Human NM_001161473.3

Homo sapiens aldehyde dehydrogenase 3 family member B1 (ALDH3B1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
ALDH3B1 (221)
Length:
2891
CDS:
148..1554

Additional Resources:

NCBI RefSeq record:
NM_001161473.3
NBCI Gene record:
ALDH3B1 (221)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001161473.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027197 GCCATCGGAGATTAGCAAGAA pLKO.1 561 CDS 100% 4.950 3.465 N ALDH3B1 n/a
2 TRCN0000027204 GATTAGCAAGAACGTCGAGAA pLKO.1 570 CDS 100% 4.050 2.835 N ALDH3B1 n/a
3 TRCN0000027208 CTGTGGGAACGACGGCTTCAT pLKO.1 1305 CDS 100% 1.650 1.155 N ALDH3B1 n/a
4 TRCN0000027162 GAGTCGGAGGTGTCTGAGGTT pLKO.1 319 CDS 100% 0.880 0.616 N ALDH3B1 n/a
5 TRCN0000027142 GCCTGGATGAAGGACGAGCGT pLKO.1 385 CDS 100% 0.000 0.000 N ALDH3B1 n/a
6 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2029 3UTR 100% 4.950 2.475 Y CFLAR n/a
7 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2029 3UTR 100% 4.950 2.475 Y C19orf31 n/a
8 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2027 3UTR 100% 4.950 2.475 Y ERN2 n/a
9 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2027 3UTR 100% 4.950 2.475 Y P3H4 n/a
10 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2027 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2196 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001161473.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00050 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00050 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478455 GGAAGACGGGCCAGGCCACACTTG pLX_317 22% 100% 100% V5 n/a
4 ccsbBroadEn_00051 pDONR223 100% 92% 92% None 162_272del n/a
5 ccsbBroad304_00051 pLX_304 0% 92% 92% V5 162_272del n/a
6 TRCN0000469174 TAAAGGAAATACTACTTACATTAT pLX_317 26.8% 92% 92% V5 162_272del n/a
7 ccsbBroadEn_05803 pDONR223 100% 71.3% 68.5% None (many diffs) n/a
8 ccsbBroad304_05803 pLX_304 0% 71.3% 68.5% V5 (many diffs) n/a
9 TRCN0000468666 TACTAGCAATCATCCCTGTGACTA pLX_317 1.3% 71.3% 68.5% V5 (many diffs) n/a
10 ccsbBroadEn_13811 pDONR223 100% 47.9% 4.5% None (many diffs) n/a
11 ccsbBroad304_13811 pLX_304 0% 47.9% 4.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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