Construct: ORF TRCN0000469185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014754.1_s317c1
- Derived from:
- ccsbBroadEn_09574
- DNA Barcode:
- ATTATTCTTTATCTTACTGATCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF183 (138065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138065 | RNF183 | ring finger protein 183 | NM_001371234.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 2 | human | 138065 | RNF183 | ring finger protein 183 | NM_001371235.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 3 | human | 138065 | RNF183 | ring finger protein 183 | NM_001371236.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 4 | human | 138065 | RNF183 | ring finger protein 183 | NM_001371237.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 5 | human | 138065 | RNF183 | ring finger protein 183 | NM_145051.4 | 99.6% | 98.9% | 244G>A;341A>G |
| 6 | human | 138065 | RNF183 | ring finger protein 183 | XM_011518218.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 7 | human | 138065 | RNF183 | ring finger protein 183 | XM_011518219.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 8 | human | 138065 | RNF183 | ring finger protein 183 | XM_017014268.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 9 | human | 138065 | RNF183 | ring finger protein 183 | XM_024447410.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 10 | human | 138065 | RNF183 | ring finger protein 183 | XM_024447411.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 11 | human | 138065 | RNF183 | ring finger protein 183 | XM_024447412.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 12 | human | 138065 | RNF183 | ring finger protein 183 | XM_024447413.1 | 99.6% | 98.9% | 244G>A;341A>G |
| 13 | mouse | 76072 | Rnf183 | ring finger protein 183 | NM_153504.3 | 85.8% | 88.5% | (many diffs) |
| 14 | mouse | 76072 | Rnf183 | ring finger protein 183 | XM_006538339.3 | 85.8% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgagcagcag ggccgggagc ttgaggctga gtgccccgtc tgctggaacc 121 ccttcaacaa cacgttccat acccccaaaa tgctggattg ctgccactcc ttctgcgtgg 181 aatgtctggc ccaccTCAGC CTTGTGACTC CAGCCCGGCG CCGCCTGCTG TGCCCACTCT 241 GTCGCCAGCC CACAGTGCTG GCCTCAGGGC AGCCTGTCAC TGACTTGCCC ACGGACACTG 301 CCATGCTCAC CCTGCTCCGC CTGGAGCCCC ACCATGTCAT CCTGGAAGGC CATCAGCTGT 361 GCCTCAAGGA CCAGCCCAAG AGCCGCTACT TCCTGCGCCA GCCTCGAGTC TACACGCTGG 421 ACCTTGGCCC CCAGCCTGGG GGCCAGACTG GGCCGCCCCC AGACACGGCC TCTGCCACCG 481 TGTCTACGCC CATCCTCATC CCCAGCCACC ACTCTTTGAG GGAGTGTTTC CGCAACCCTC 541 AGTTCCGCAT CTTTGCCTAC CTGATGGCCG TCATCCTCAG TGTCACTCTG TTGCTCATAT 601 TCTCCATCTT TTGGACCAAG CAGTTCCTTT GGGGTGTGGG GTGCCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAATT ATTCTTTATC TTACTGATCA AACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t