Construct: ORF TRCN0000469210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010916.1_s317c1
- Derived from:
- ccsbBroadEn_09138
- DNA Barcode:
- CCAGCCGATGCGGACATCGAATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFI27L2 (83982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83982 | IFI27L2 | interferon alpha inducible ... | NM_032036.3 | 98.7% | 98.4% | 13_16delGCAGinsA;372G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGAT GAAACGGACT GCTGCTGCAG TGGGAGGAGC CCTGGCAGTG GGGGCTGTGC 121 CCGTGGTGCT CAGTGCCATG GGCTTCACTG GGGCAGGAAT CGCCGCGTCC TCCATAGCAG 181 CCAAGATGAT GTCCGCAGCA GCCATTGCCA ACGGGGGTGG TGTTTCTGCG GGGAGCCTGG 241 TGGCTACTCT GCAGTCCGTG GGGGCAGCTG GACTCTCCAC ATCATCCAAC ATCCTCCTGG 301 CCTCTGTTGG GTCAGTGTTG GGGGCCTGCT TGGGGAATTC ACCTTCTTCT TCTCTCCCAG 361 CTGAACCCGA GGCTAAAGAA GATGAGGCAA GAGAAAATGT ACCCCAAGGT GAACCTCCAA 421 AACCCCCACT CAAATCAGAG AAACATGAGG AATACCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 601 AGCCGATGCG GACATCGAAT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt