Construct: ORF TRCN0000469222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010667.1_s317c1
- Derived from:
- ccsbBroadEn_12643
- DNA Barcode:
- AATTGCGGAGTACAACATACCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGIX (79917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_005278064.4 | 77.8% | 73.9% | 502_516del;775_776insG;825_826ins215 |
| 2 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_005278065.3 | 77.8% | 73.9% | 502_516del;775_776insG;825_826ins215 |
| 3 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_011543985.2 | 77.8% | 73.9% | 502_516del;775_776insG;825_826ins215 |
| 4 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_011543986.2 | 77.8% | 73.9% | 502_516del;775_776insG;825_826ins215 |
| 5 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_011543987.2 | 77.8% | 73.9% | 502_516del;775_776insG;825_826ins215 |
| 6 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_005278063.4 | 64.9% | 54.6% | (many diffs) |
| 7 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_005278062.4 | 64.1% | 53.9% | (many diffs) |
| 8 | human | 79917 | MAGIX | MAGI family member, X-linked | XM_006724560.3 | 62.7% | 53.4% | (many diffs) |
| 9 | human | 79917 | MAGIX | MAGI family member, X-linked | NM_024859.3 | 61.9% | 52.7% | (many diffs) |
| 10 | human | 79917 | MAGIX | MAGI family member, X-linked | NM_001099682.2 | 57.2% | 41.7% | (many diffs) |
| 11 | human | 79917 | MAGIX | MAGI family member, X-linked | NM_001099681.2 | 56.4% | 41.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1095
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccactattg tggatcaccg gccccaggta ccatctcatc cttctatcgg 121 aggcctcctg cctcagggca aactatgtac acctgtgtcc tttattccag caccgttggt 181 tagagacatg taacgcacct ccccaattga tccagggtaa ggcacgtagt gctccgaagc 241 catcccaggc ctctggtcat ttctctgtgg agctggttcg cggttacgca ggctttggcc 301 tcaccttagg tgggggccgg gatgtagctg gggacactcc gctggccgtg cgcgggctgc 361 tgaaggatgg cccagcacag cgctgtggtc gtttggaggt cggggacctc gtgctccaca 421 tcaacggaga gtcaacgcag ggcctcaccc atgcccaggc cgtggagcgg atccgagctg 481 gaggccccca gctccacctg gttattcgtc ggcctctgga gacccaccct ggcaagcctc 541 gaggggtggg agagccccga aaaggagttg atcgcagccc agatcctgga gggccggagg 601 taacggggtc tcgcagcagc agcacttccc tagttcagca ccctccatcc cggacgacgc 661 tcaagaagac ccggggcagc ccggagccta gtccagaggc ggccgccgat ggccccacgg 721 tttctcctcc TGAGCGCCGC GCTGAGGATC CCAACGACCA GATCCCGGGT TCCCCGGGGC 781 CCTGGCTAGT GCCCAGCGAG GAACGGCTCT CGCGGGCCCT AGGGGTCCGG GGGGGCAGCG 841 CAGCTCGCTC AGGAGATGGC AGCCGGAAGG CGGAGACACT GAGCCTACCT CTGACAGCGC 901 GGGGCTCACT AGTTACCGCC CAACCTGGAT CCGGCGCGTG TGGCCGCTGG GCACTTGGCA 961 CCTCCCCAAG AGCTTCCACT TGGTTTGGTC CCCCCACCCG CTGCTCAGTG GCTCTGACCA 1021 CACTCCCCTC GCAGCGTCAG TGGTACGACC CTTCCAGCCG GCTAGCCTGC GCTTCTGGTT 1081 CTGGGAGGGG GAGGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATTGCGGAG TACAACATAC 1261 CCGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt