Construct: ORF TRCN0000469242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016708.1_s317c1
- Derived from:
- ccsbBroadEn_06996
- DNA Barcode:
- ACGCACCCGAACGAATAGTAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRM (6723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6723 | SRM | spermidine synthase | NM_003132.3 | 99.8% | 100% | 102A>G |
| 2 | human | 6723 | SRM | spermidine synthase | XM_017002179.2 | 82.8% | 80.6% | (many diffs) |
| 3 | mouse | 20810 | Srm | spermidine synthase | NM_009272.4 | 88.9% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccggcccc gacggccccg ccgcctccgg ccccgccgcc atccgcgagg 121 gctggttccg cgagacctgc agcctgtggc ccggccaggc cctgtcgctg caggtggagc 181 agctgctcca ccaccggcgc tcgcgctacc aggacatcct cgtcttccgc agtaagacct 241 atggcaacgt gctggtgttg gacggtgtca tccagtgcac ggagagagac gagttctcct 301 accaggagat gatcgccaac ctgcctctct gcagccaccc caacccgcga aaggtgctga 361 tcatcggggg cggagatgga ggtgtcctgc gggaggtggt gaagcacccc tccgtggagt 421 ccgtggtcca gtgtgagatc gacgaggatg tcatccaagt ctccaagaag ttcctgccag 481 gcatggccat tggctactct agctcgaagc tgaccctaca tgtgggtgac ggttttgagt 541 tcatgaaaca gaatcaggat gccttcgacg tgatcatcac tgactccTCA GACCCCATGG 601 GCCCCGCCGA AAGTCTCTTC AAGGAGTCCT ATTACCAGCT CATGAAGACA GCCCTCAAGG 661 AAGATGGTGT CCTCTGCTGC CAGGGCGAGT GCCAGTGGCT GCACCTGGAC CTCATCAAGG 721 AGATGCGGCA GTTCTGCCAG TCCCTGTTCC CCGTGGTGGC CTATGCCTAC TGCACCATCC 781 CCACCTACCC CAGCGGCCAG ATCGGCTTCA TGCTGTGCAG CAAGAACCCG AGCACGAACT 841 TCCAGGAGCC GGTGCAGCCG CTGACACAGC AGCAGGTGGC GCAGATGCAG CTGAAGTACT 901 ACAACTCCGA CGTGCACCGC GCCGCCTTTG TGCTGCCCGA GTTTGCCCGC AAGGCCCTGA 961 ATGATGTGAG CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACG CACCCGAACG AATAGTAGTA 1141 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t