Transcript: Human NM_003132.3

Homo sapiens spermidine synthase (SRM), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
SRM (6723)
Length:
1260
CDS:
76..984

Additional Resources:

NCBI RefSeq record:
NM_003132.3
NBCI Gene record:
SRM (6723)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149020 GGACGGTGTCATCCAGTGCA pXPR_003 CGG 211 23% 2 1.0281 SRM SRM 76280
2 BRDN0001147064 ACTGACTCCTCAGACCCCAT pXPR_003 GGG 530 58% 4 0.7048 SRM SRM 76279
3 BRDN0001147759 CTCGAAGCTGACCCTACATG pXPR_003 TGG 454 50% 4 0.1153 SRM SRM 76281
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003132.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045728 AGTCTCTTCAAGGAGTCCTAT pLKO.1 622 CDS 100% 4.950 3.465 N SRM n/a
2 TRCN0000290713 AGTCTCTTCAAGGAGTCCTAT pLKO_005 622 CDS 100% 4.950 3.465 N SRM n/a
3 TRCN0000045729 CATCCAAGTCTCCAAGAAGTT pLKO.1 462 CDS 100% 4.950 3.465 N SRM n/a
4 TRCN0000290784 CATCCAAGTCTCCAAGAAGTT pLKO_005 462 CDS 100% 4.950 3.465 N SRM n/a
5 TRCN0000045732 CTTCATGCTGTGCAGCAAGAA pLKO.1 816 CDS 100% 4.950 3.465 N SRM n/a
6 TRCN0000290715 CTTCATGCTGTGCAGCAAGAA pLKO_005 816 CDS 100% 4.950 3.465 N SRM n/a
7 TRCN0000045731 CATTGGCTACTCTAGCTCGAA pLKO.1 498 CDS 100% 2.640 1.848 N SRM n/a
8 TRCN0000290714 CATTGGCTACTCTAGCTCGAA pLKO_005 498 CDS 100% 2.640 1.848 N SRM n/a
9 TRCN0000045730 GCCTTTGTGCTGCCCGAGTTT pLKO.1 934 CDS 100% 1.650 0.990 N SRM n/a
10 TRCN0000290783 GCCTTTGTGCTGCCCGAGTTT pLKO_005 934 CDS 100% 1.650 0.990 N SRM n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003132.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06996 pDONR223 100% 99.8% 100% None 102A>G n/a
2 ccsbBroad304_06996 pLX_304 0% 99.8% 100% V5 102A>G n/a
3 TRCN0000469242 ACGCACCCGAACGAATAGTAGTAA pLX_317 42.6% 99.8% 100% V5 102A>G n/a
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