Construct: ORF TRCN0000469245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011777.1_s317c1
- Derived from:
- ccsbBroadEn_01889
- DNA Barcode:
- TAGTTTTCCCTGTCTCAATACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FZD4 (8322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8322 | FZD4 | frizzled class receptor 4 | NM_012193.4 | 100% | 100% | |
| 2 | mouse | 14366 | Fzd4 | frizzled class receptor 4 | NM_008055.4 | 92.3% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1680
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctggcgg ggcgcagggc cgagcgtccc gggggcgccc gggggcgtcg 121 gtctcagtct ggggttgctc ctgcagttgc tgctgctcct ggggccggcg cggggcttcg 181 gggacgagga agagcggcgc tgcgacccca tccgcatctc catgtgccag aacctcggct 241 acaacgtgac caagatgccc aacctggttg ggcacgagct gcagacggac gccgagctgc 301 agctgacaac tttcacaccg ctcatccagt acggctgctc cagccagctg cagttcttcc 361 tttgttctgt ttatgtgcca atgtgcacag agaagatcaa catccccatt ggcccatgcg 421 gcggcatgtg tctttcagtc aagagacgct gtgaacccgt cctgaaggaa tttggatttg 481 cctggccaga gagtctgaac tgcagcaaat tcccaccaca gaacgaccac aaccacatgt 541 gcatggaagg gccaggtgat gaagaggtgc ccttacctca caaaaccccc atccagcctg 601 gggaagagtg tcactctgtg ggaaccaatt ctgatcagta catctgggtg aaaaggagcc 661 tgaactgtgt gctcaagtgt ggctatgatg ctggcttata cagccgctca gccaaggagt 721 tcactgatat ctggatggct gtgtgggcca gcctgtgttt catctccact gccttcacag 781 tactgacctt cctgatcgat tcttctaggt tttcctaccc tgagcgcccc atcatatttc 841 tcagtatgtg ctataatatt tatagcattg cttatattgt caggctgact gtaggccggg 901 aaaggatatc ctgtgatttt gaagaggcag cagaacctgt tctcatccaa gaaggactta 961 agaacacagg atgtgcaata attttcttgc tgatgtactt ttttggaatg gccagctcca 1021 tttggtgggt tattctgaca ctcacttggt ttttggcagc aggactcaaa tggggtcatg 1081 aagccattga aatgcacagc tcttatttcc acattgcagc ctgggccatc cccgcagtga 1141 aaaccattgt catcttgatt atgagactgg tggatgcaga tgaactgact ggcttgtgct 1201 atgttggaaa ccaaaaTCTC GATGCCCTCA CCGGGTTCGT GGTGGCTCCC CTCTTTACTT 1261 ATTTGGTCAT TGGAACTTTG TTCATTGCTG CAGGTTTGGT GGCCTTGTTC AAAATTCGGT 1321 CAAATCTTCA AAAGGATGGG ACAAAGACAG ACAAGTTAGA AAGACTGATG GTCAAGATTG 1381 GGGTGTTCTC AGTACTGTAC ACAGTTCCTG CAACGTGTGT GATTGCCTGT TATTTTTATG 1441 AAATCTCCAA CTGGGCACTT TTTCGGTATT CTGCAGATGA TTCCAACATG GCTGTTGAAA 1501 TGTTGAAAAT TTTTATGTCT TTGTTGGTGG GCATCACTTC AGGCATGTGG ATTTGGTCTG 1561 CCAAAACTCT TCACACGTGG CAGAAGTGTT CCAACAGATT GGTGAATTCT GGAAAGGTAA 1621 AGAGAGAGAA GAGAGGAAAT GGTTGGGTGA AGCCTGGAAA AGGCAGTGAG ACTGTGGTAT 1681 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGATAGTT TTCCCTGTCT CAATACCCTA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt