Construct: ORF TRCN0000469250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008349.1_s317c1
- Derived from:
- ccsbBroadEn_02878
- DNA Barcode:
- GAACATGTGATCCGCGTTGTGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POC1A (25886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25886 | POC1A | POC1 centriolar protein A | NM_015426.5 | 100% | 100% | |
| 2 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533560.1 | 91.8% | 91.8% | 882_883ins99 |
| 3 | human | 25886 | POC1A | POC1 centriolar protein A | NM_001161581.2 | 90.6% | 90.6% | 0_1ins114 |
| 4 | human | 25886 | POC1A | POC1 centriolar protein A | NM_001161580.2 | 88.2% | 88.2% | 980_981ins144 |
| 5 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533561.1 | 80% | 80% | 882_883ins243 |
| 6 | human | 25886 | POC1A | POC1 centriolar protein A | XM_017006104.1 | 78.8% | 78.8% | 0_1ins114;866_867ins144 |
| 7 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533562.2 | 73.4% | 72.4% | (many diffs) |
| 8 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533563.1 | 60.4% | 55% | 679_680ins134;738_739ins349 |
| 9 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533564.3 | 60.4% | 55% | 679_680ins134;738_739ins349 |
| 10 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533565.2 | 60.4% | 55% | 679_680ins134;738_739ins349 |
| 11 | human | 25886 | POC1A | POC1 centriolar protein A | XR_940402.1 | 57.2% | 1_40del;719_720ins134;1128_1764del | |
| 12 | human | 25886 | POC1A | POC1 centriolar protein A | XR_001740088.1 | 57% | 1_40del;1021_1262del;1504_2140del | |
| 13 | human | 25886 | POC1A | POC1 centriolar protein A | XR_940403.1 | 49.6% | (many diffs) | |
| 14 | mouse | 70235 | Poc1a | POC1 centriolar protein A | NM_027354.2 | 88.7% | 90.4% | (many diffs) |
| 15 | mouse | 70235 | Poc1a | POC1 centriolar protein A | XM_006511830.3 | 78.8% | 73.6% | (many diffs) |
| 16 | mouse | 70235 | Poc1a | POC1 centriolar protein A | XM_006511831.3 | 76.6% | 75.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1290
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcgccc tgcgcggagg acccctcgct ggaaaggcat tttaagggcc 121 accgagatgc agttacctgt gtggacttca gtatcaacac aaagcagctg gccagtggct 181 ccatggactc atgcctcatg gtctggcaca tgaagccgca gtcacgcgcc taccgcttca 241 ctggccacaa ggatgccgtc acctgtgtga acttctctcc ttcgggacac ctgcttgctt 301 ccggctcccg agacaagact gtccgcatct gggtacccaa tgtcaaaggt gagtccactg 361 tgtttcgtgc acacacagcc acagtgagga gtgtccactt ctgcagtgat ggccagtcct 421 tcgtgacagc ctctgacgac aagacagtca aagtgtgggc aactcatcgc cagaaattcc 481 tgttctccct gagccagcat atcaactggg tccgctgtgc caagttctcc cccgacgggc 541 ggctcatcgt gtctgccagt gatgacaaga ctgttaagct gtgggacaag agcagccggg 601 aatgtgtcca ctcgtattgt gagcatggcg gctttgtcac ctatgtggac ttccacccca 661 gtgggacgtg cattgccgct gccggcatgg acaacacagt gaaggtgtgg gacgtgcgga 721 ctcaccggct gctgcagcat tatcagttgc acagtgcagc agtgaacggg ctctctttcc 781 acccgtcggg aaactacctg atcacagcct ccagtgactc aaccctgaag atcctggacc 841 TGATGGAGGG CCGGCTGCTC TACACACTCC ACGGGCATCA GGGACCAGCC ACCACTGTTG 901 CCTTTTCAAG AACGGGGGAG TATTTTGCTT CTGGAGGCTC TGATGAACAA GTGATGGTTT 961 GGAAGAGTAA CTTTGATATT GTTGATCATG GAGAAGTCAC GAAAGTGCCG AGGCCCCCAG 1021 CCACACTGGC CAGCTCCATG GGGAATCTGC CAGAAGTGGA CTTCCCTGTC CCCCCAGGCA 1081 GAGGCAGGAG TGTGGAGTCT GTGCAGAGCC AGCCCCAGGA GCCCGTGAGT GTGCCCCAGA 1141 CACTGACTAG CACGCTGGAG CACATTGTGG GCCAGCTGGA TGTCCTCACT CAGACAGTCT 1201 CCATTCTGGA GCAGCGGTTG ACACTGACAG AAGACAAGCT GAAGCAGTGT CTGGAGAACC 1261 AGCAGCTAAT CATGCAGAGA GCAACACCAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAACA 1441 TGTGATCCGC GTTGTGCGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt