Construct: ORF TRCN0000469254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015952.1_s317c1
- Derived from:
- ccsbBroadEn_00190
- DNA Barcode:
- TCTGCCGCAATCGCGGGCAAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9 (735)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 735 | C9 | complement C9 | NM_001737.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1743
- ORF length:
- 1677
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agcctgccgg agctttgcag ttgcaatctg cattttagaa ataagcatcc 121 tcacagcaca gtacacgacc agttatgacc cagagctaac agaaagcagt ggctctgcat 181 cacacataga ctgcagaatg agcccctgga gtgaatggtc acaatgcgat ccttgtctca 241 gacaaatgtt tcgttcaaga agcattgagg tctttggaca atttaatggg aaaagatgca 301 ccgacgctgt gggagacaga cgacagtgtg tgcccacaga gccctgtgag gatgctgagg 361 atgactgcgg aaatgacttt caatgcagta caggcagatg cataaagatg cgacttcggt 421 gtaatggtga caatgactgc ggagactttt cagatgagga tgattgtgaa agtgagcccc 481 gtcccccctg cagagacaga gtggtagaag agtctgagct ggcacgaaca gcaggctatg 541 ggatcaacat tttagggatg gatcccctaa gcacaccttt tgacaatgag ttctacaatg 601 gactctgtaa ccgggatcgg gatggaaaca ctctgacata ctaccgaaga ccttggaacg 661 tggcttcttt gatctatgaa accaaaggcg agaaaaattt cagaaccgaa cattacgaag 721 aacaaattga agcatttaaa agtatcatcc aagagaagac atcaaatttt aatgcagcta 781 tatctctaaa atttacaccc actgaaacaa ataaagctga acaatgttgt gaggaaacag 841 cctcctcaat ttctttacat ggcaagggta gttttcggtt ttcatattcc aaaaatgaaa 901 cttaccaact atttttgtca tattcttcaa agaaggaaaa aatgtttctg catgtgaaag 961 gagaaattca tctgggaaga tttgtaatga gaaatcgcga tgttgtgctc acaacaactt 1021 ttgtggatga tataaaagct ttgccaacta cctatgaaaa gggagaatat tttgcctttt 1081 tggaaaccta tggaactcac tacagtagct ctgggtctct aggaggactc tatgaactaa 1141 tatatgtttt ggataaagct tccatgaagc ggaaaggtgt tgaactaaaa gacataaaga 1201 gatgccttgg gtatcatctg gatgtatctc tggctttctc tgaaatctct gttggagctg 1261 aatttaataa agatgattgt gtaaagaggg gagagggtag agctgtaaac atcaccagtg 1321 aaaaccTCAT AGATGATGTT GTTTCACTCA TAAGAGGTGG AACCAGAAAA TATGCATTTG 1381 AACTGAAAGA AAAGCTTCTC CGAGGAACCG TGATTGATGT GACTGACTTT GTCAACTGGG 1441 CCTCTTCCAT AAATGATGCT CCTGTTCTCA TTAGTCAAAA ACTGTCTCCT ATATATAATC 1501 TGGTTCCAGT GAAAATGAAA AATGCACACC TAAAGAAACA AAACTTGGAA AGAGCCATTG 1561 AAGACTATAT CAATGAATTT AGTGTAAGAA AATGCCACAC ATGCCAAAAT GGAGGTACAG 1621 TGATTCTAAT GGATGGAAAG TGTTTGTGTG CCTGCCCATT CAAATTTGAG GGAATTGCCT 1681 GTGAAATCAG TAAACAAAAA ATTTCTGAAG GATTGCCAGC CCTAGAGTTC CCCAATGAAA 1741 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGATC TGCCGCAATC GCGGGCAAAT GAACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt