Construct: ORF TRCN0000469266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000453.1_s317c1
- Derived from:
- ccsbBroadEn_16149
- DNA Barcode:
- ACCAGGCGGTGCCAGTCAGCATAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EOGT (285203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285203 | EOGT | EGF domain specific O-linke... | NR_103826.2 | 9.4% | 1_2340del;2726_2728delTATinsA;2746_4232del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ctcagctcac tgcaacctct gccttctgga ttcaagtgat tcttgtgcct 121 tagccTCCCA AGTAGCTGGG ATTACAGGCG TGCACCACCA CGCCCAGTTG ATTTTTGTAT 181 TTTTGATAGA GACGGAGTTT CACCATGTTG GCCAGGCTGG TCTCGAACTC TGGGTTCAAG 241 AAATCCTCCC ACCTTGCCTC CCAAAGTGCT GGGATTACAG GTGTGAGCCA CCACGCATGG 301 CCCTGAACTT TCTCTTTTTA GGAATACCAA AGTTTTCAAC TTTTTCAGCT TTAGAATTTG 361 TAAATATTTT TGTAGAATAT CATATGACTG TAATTCCAGA GTGTTCCAAC TTGTTTATGA 421 TATATTTGGG TAAATTTACA ACTGTTCTTT ATTGCCATAA TCTGGTTATA CCCAACTTTC 481 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 541 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 601 TGTGGAAAGG ACGAACCAGG CGGTGCCAGT CAGCATATAC GCGTTAAGTC gacaatcaac 661 ctctggatta caaaatttgt gaaagatt