Construct: ORF TRCN0000469309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004059.1_s317c1
- Derived from:
- ccsbBroadEn_16032
- DNA Barcode:
- ATTCTAACCATGTCAATTAAATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MCRIP2 (84331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_001331229.2 | 52.8% | 50% | (many diffs) |
| 2 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_138418.4 | 46.6% | 39.3% | 181_308del;343G>A;354_480del |
| 3 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138606.2 | 27.8% | 1_164del;379G>A;390_803del | |
| 4 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138608.2 | 20.2% | (many diffs) | |
| 5 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138604.2 | 20.1% | (many diffs) | |
| 6 | human | 286467 | FIRRE | firre intergenic repeating ... | NR_026975.2 | 6.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 237
- ORF end:
- 450
- ORF length:
- 213
- Sequence:
-
1 GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA 61 GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA 121 GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT 181 TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGT 241 ACACCATCAC CAAGGGGCCC AGCAAGCTGG TCGCGCAGCG CCGCACAGGT CCCACGCAGC 301 AGCAGGTGGA GGGCCGGCTC GGCGAGCTCC TGAAATGCCG GCAGCCCGCG CCGCCGACCT 361 CGCAGCCCCC GCGGGCGCAG CCCTTTGCGC AGCCGCCGGG ACCCTGGCCC CTGTCGAGCC 421 TGGCAGCAGG TGCAACAGCA GCTGGATGGT AGCCCAGCCG GTGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGAATT CTAACCATGT CAATTAAATC TACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t