Construct: ORF TRCN0000469315
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009019.1_s317c1
- Derived from:
- ccsbBroadEn_07147
- DNA Barcode:
- GGACTCACAACCGATTTTTTACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF8 (7554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469315
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7554 | ZNF8 | zinc finger protein 8 | NM_021089.3 | 99.9% | 100% | 1512A>G |
| 2 | human | 108903150 | ZNF8-ERVK3-1 | ZNF8-ERVK3-1 readthrough | NR_144447.1 | 36.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccccgaggac gaaggggtag cgggagtgat gtctgtgggg ccgccggcgg 121 cccggcttca ggaaccagtg accttccggg atgtggctgt ggactttacc caggaggaat 181 gggggcagct ggaccctacc cagaggatcc tctaccgtga cgtgatgctg gagacctttg 241 gtcacctgct ctccataggt cctgagcttc cgaagcctga agtcatctcc cagctggagc 301 aagggaccga gctatgggtg gctgagagag gaaccaccca gggctgccat ccagcctggg 361 agcctcgatc tgaaagccaa gcatcacgca aggaagaggg cctgcctgaa gaggagccat 421 cccatgtcac gggaagggaa ggattcccga cagatgctcc ttatcccacc acgttaggga 481 aagacaggga gtgtcagagc cagagtctgg cactcaagga gcagaataac ttgaagcagt 541 tggaatttgg cctcaaggaa gcaccagttc aagatcaagg ctacaaaact ctcagactca 601 gggaaaactg cgtcctgagt tcaagcccaa atccattccc agagatctct agaggggagt 661 atttgtatac ttacgactca cagattacag actcagaaca taactccagc ttagtcagtc 721 agcagacagg ctccccagga aaacagcccg gtgaaaacag tgactgtcac agagattcca 781 gtcaggccat tccaattacg gaactcacaa aaagccaggt gcaggacaaa ccctacaaat 841 gtactgactg tgggaagtcg tttaaccata acgcacacct caccgtgcac aagaggattc 901 atacgggaga aagaccttat atgtgcaagg agtgtgggaa agccttcagc cagaactcct 961 ccctcgtcca gcatgagcgc atccacactg gagacaagcc ctacaagtgt gccgaatgtg 1021 ggaagtcttt ctgccatagt acacacctta ccgtccatcg gaggattcac actggggaga 1081 agccctatga gtgtcaggac tgtgggaggg ccttcaacca gaactcctcc ctggggcggc 1141 acaagaggac acacactggg gagaagccat acacctgcag tgtgtgtggg aaatccttct 1201 ctcggaccac ttgccttttc ctgcacctga gaactcacac cgaggagagg ccctacgagt 1261 gtaaccactg cgggaagggc ttcaggcaca gctcatccct ggcccagcac cagcggaagc 1321 acgcggggga gaagcccttt gagtgccgcc agaggctgat ctttGAGCAG ACGCCAGCTC 1381 TCACAAAGCA TGAATGGACA GAAGCCCTGG GCTGTGACCC ACCTTTGAGT CAAGATGAGA 1441 GGACTCACCG AAGCGACAGA CCCTTCAAAT GTAATCAGTG TGGGAAGTGT TTCATTCAGA 1501 GCTCTCACCT CATCCGGCAC CAGATAACTC ACACCAGAGA GGAGCAGCCC CATGGGCGAA 1561 GCCGGCGGCG TGAACAGTCC TCGAGCAGGA ACTCACACCT GGTTCAGCAT CAACACCCGA 1621 ACTCCAGAAA GAGCTCTGCA GGCGGAGCAA AGGCAGGGCA GCCGGAAAGC AGAGCCCTGG 1681 CTTTGTTTGA CATCCAAAAA ATCATGCAAG AGAAAAACCC TGTGCACGTT ATTGGGGTGG 1741 AAGAGCCTTC TGTGGGTGCT TCCATGTTAT TTGACATCAG AGAATCCACA TACCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGAGGAC TCACAACCGA TTTTTTACAC ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt