Construct: ORF TRCN0000469318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018010.1_s317c1
- Derived from:
- ccsbBroadEn_06118
- DNA Barcode:
- GATTTCTTCTTGCTTTCGCTATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPYSL3 (1809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | NM_001387.3 | 99.8% | 99.8% | 227T>A;288C>T;1629C>T |
| 2 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | XM_011537574.2 | 98.4% | 97% | (many diffs) |
| 3 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | NM_001197294.2 | 82.5% | 81.8% | (many diffs) |
| 4 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_009468.5 | 90.6% | 98.2% | (many diffs) |
| 5 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_001136086.2 | 89.7% | 96.4% | (many diffs) |
| 6 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_001291455.1 | 75% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1776
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctaccaaggc aagaagaaca tcccgcggat cacgagtgac cgtctcctta 121 tcaagggagg cagaatcgtc aatgatgatc agtcctttta tgctgatatt tacatggaag 181 atggcttaat aaaacaaatt ggagacaatc tgattgttcc tggaggagtg aagaccattg 241 aagccaatgg gaagatggtg atccctggag gcatcgatgt ccatactcac taccagatgc 301 catataaggg aatgaccaca gtagatgact tcttccaagg gacaaaggcg gctttagcag 361 gtggcaccac catgatcatt gaccatgtgg tgcctgagcc tgagtccagc ctgactgagg 421 cctatgagaa atggagagag tgggctgatg ggaagagttg ctgtgactat gccctgcatg 481 tggacatcac ccactggaat gacagcgtca agcaggaagt gcagaacctc atcaaggaca 541 aaggggttaa ctccttcatg gtttatatgg cttataagga tttgtatcaa gtatctaaca 601 cagagctcta tgagatcttc acctgcctgg gagagctggg ggccattgct caagttcatg 661 ctgagaatgg ggatatcatt gcccaggagc aaacccgcat gttggaaatg gggataactg 721 gcccagaagg ccatgtactg agcaggccag aagagctgga agctgaggct gtgttccgtg 781 ccatcaccat tgccagccaa accaattgcc ctctctacgt cacaaaggtc atgagcaaga 841 gtgcagctga cctcatctca caagccagga aaaaaggaaa tgtagtcttt ggtgagccca 901 tcactgccag cctcggcata gatggaaccc attattggag caagaactgg gccaaggcgg 961 ctgcatttgt gacatcccca cccctgagcc ctgacccaac tactccggac tacatcaact 1021 ccttgctggc cagcggggat ctgcagctat ctgggagtgc ccactgcacc ttcagcactg 1081 cccagaaagc aattgggaag gacaacttca cagccattcc tgagggcacc aatggtgtgg 1141 aggagcggat gtctgtcatc tgggacaagg ctgtggccac agggaaaatg gacgaaaacc 1201 agttcgtggc tgtgacaagc acaaacgctg ccaagatctt caacctgtat ccccgcaagg 1261 gaagaatatc tgtgggttct gacagcgacc tcgtcatctg ggatccagat gctgtgaaga 1321 tcgtctctgc caagaaccac cagtctgcgg cagagtacaa catctttgaa gggatggagc 1381 tgcgcggGGC TCCTCTGGTT GTCATCTGCC AGGGCAAGAT CATGCTGGAA GATGGCAACC 1441 TGCACGTGAC CCAGGGGGCT GGCCGCTTCA TACCCTGCAG CCCGTTCTCC GACTATGTCT 1501 ACAAGCGCAT TAAAGCACGG AGGAAGATGG CAGACCTGCA TGCCGTCCCA AGGGGCATGT 1561 ACGATGGGCC TGTGTTTGAC CTGACCACCA CCCCCAAAGG TGGCACCCCC GCAGGCTCTG 1621 CTCGGGGCTC TCCTACTCGG CCGAACCCAC CTGTGAGGAA TCTTCATCAG TCGGGATTTA 1681 GCCTGTCAGG CACTCAAGTG GATGAGGGGG TTCGCTCAGC CAGCAAGCGC ATCGTGGCGC 1741 CCCCAGGCGG CCGTTCTAAT ATCACATCTC TGAGTTACCC AACTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 AGATTTCTTC TTGCTTTCGC TATTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt