Construct: ORF TRCN0000469324
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015482.1_s317c1
- Derived from:
- ccsbBroadEn_08235
- DNA Barcode:
- AGCTTGTGTCGATATTCCGTCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C3orf18 (51161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469324
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51161 | C3orf18 | chromosome 3 open reading f... | NM_001171740.3 | 99.7% | 99.3% | 485C>T |
| 2 | human | 51161 | C3orf18 | chromosome 3 open reading f... | NM_001171741.2 | 99.7% | 99.3% | 485C>T |
| 3 | human | 51161 | C3orf18 | chromosome 3 open reading f... | NM_016210.5 | 99.7% | 99.3% | 485C>T |
| 4 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533782.2 | 97.3% | 96.9% | 234_245del;497C>T |
| 5 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533783.2 | 97.3% | 96.9% | 234_245del;497C>T |
| 6 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533784.2 | 97.3% | 96.9% | 234_245del;497C>T |
| 7 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533785.2 | 97.3% | 96.9% | 234_245del;497C>T |
| 8 | human | 51161 | C3orf18 | chromosome 3 open reading f... | NM_001171743.3 | 87.4% | 87% | 174_175ins60;425C>T |
| 9 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533790.1 | 87.4% | 87% | 174_175ins60;425C>T |
| 10 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_017006547.1 | 87.4% | 87% | 174_175ins60;425C>T |
| 11 | human | 51161 | C3orf18 | chromosome 3 open reading f... | XM_011533781.2 | 58.7% | 34.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctccaggacc gcatctgcta ggggctggtt cagcagccgc ccacccacct 121 ctgagtctga cctggaacct gccacagatg ggccagcctc cgagacCACT ACCCTCAGCC 181 CAGAGGCCAC CACCTTTAAT GACACCAGAA TCCCTGATGC AGCTGGTGGC ACGGCCGGCG 241 TGGGTACCAT GCTTCTGTCC TTTGGGATCA TCACGGTGAT AGGCCTGGCT GTGGCCTTGG 301 TTTTGTACAT CAGGAAGAAG AAGAGGCTGG AGAAGCTACG CCACCAGCTC ATGCCCATGT 361 ACAACTTCGA CCCCACGGAG GAACAAGATG AGTTGGAGCA GGAGCTGCTG GAGCATGGGC 421 GGGACGCCGC CTCTGTACAG GCTGCTACTT CTGTGCAGGC CATGCAGGGC AAGACTACTC 481 TGCCCTCCCA GGGCCCACTG CAGAGACCCA GCCGGCTGGT GTTTACCGAT GTGGCCAATG 541 CCATCCATGT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC TTGTGTCGAT ATTCCGTCGA 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t