Construct: ORF TRCN0000469358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016104.1_s317c1
- Derived from:
- ccsbBroadEn_14319
- DNA Barcode:
- CAGATGGTATTGCAACCGCGGCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RNFT2 (84900)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469358
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84900 | RNFT2 | ring finger protein, transm... | NM_032814.4 | 47.5% | 35.4% | (many diffs) |
| 2 | human | 84900 | RNFT2 | ring finger protein, transm... | NM_001109903.1 | 45% | 33.6% | (many diffs) |
| 3 | human | 84900 | RNFT2 | ring finger protein, transm... | XR_001748905.2 | 43% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgcgtccagg gaggaaggag ggggccgggg cgaggggggc gcctaccacc 121 accgccagcc ccaccaccat ttccaccatg gcggccaccg cgggggctcc ctgctgcagc 181 acgtgggtgg ggaccaccgg gggcactcgg aggagggagg cgacgagcag cctgggacgc 241 ccgcccccgc cctgtccgag ctgaaggctg tgatctgctg gctccagaaa ggactcccct 301 tcatcctgat cctcctggcc aaactgtgct ttcagcataa gctcggcatt gctgtgtgca 361 tcgggatggc cagcaccTTC GCCTATGCCA ACTCCACGCT TCGAGAACAG GTCTCACTGA 421 AGGAGAAGAG GTCAGTGCTG GTCATCTTGT GGATCCTGGC CTTTCTGGCG GGGAACACCC 481 TCTATGTGCT TTATACATTC AGCTCCGAGA TTGCGCCACA GCACTCCAAC CTGGGTGACA 541 GAGTGAGTGA GACTCTGTCT AAAAAAAAAA GAAAGAAAGA AGGGAGGGAG GGAGGAAGGA 601 AGGAAGGAAG GAAAGAAAGA AAGCTCAGCT TTGGCCCAGA TGTGGTTACC CCTTGGTCTC 661 CTGTCTTTAT GTCTTTCTCC TCTTCCTATT CTGTCATCTC CCTCACTTAA GTCTCAGGCC 721 TGTCAGCAGC TCCTGTGGAC ATTGCCATCC CCTCTGGTAC CCAACTTTCT TGTACAAAGT 781 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 841 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 901 CGACAGATGG TATTGCAACC GCGGCCGACG CGTTAAGTCg acaatcaacc tctggattac 961 aaaatttgtg aaagatt