Construct: ORF TRCN0000469359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016415.1_s317c1
- Derived from:
- ccsbBroadEn_15974
- DNA Barcode:
- CAACTGTCTGTAAAGCGAATCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQCH (64799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64799 | IQCH | IQ motif containing H | NM_001284349.1 | 74% | 74% | 1_237del |
| 2 | human | 64799 | IQCH | IQ motif containing H | NM_001322472.2 | 38% | 36.6% | (many diffs) |
| 3 | human | 64799 | IQCH | IQ motif containing H | NM_001322471.2 | 37.6% | 36.1% | (many diffs) |
| 4 | human | 64799 | IQCH | IQ motif containing H | NM_001284348.2 | 35.4% | 34.1% | (many diffs) |
| 5 | human | 64799 | IQCH | IQ motif containing H | NM_001322473.2 | 33.6% | 32.3% | (many diffs) |
| 6 | human | 64799 | IQCH | IQ motif containing H | NM_001322474.2 | 33.5% | 32.2% | (many diffs) |
| 7 | human | 64799 | IQCH | IQ motif containing H | NM_001284347.2 | 32.2% | 30.9% | (many diffs) |
| 8 | human | 64799 | IQCH | IQ motif containing H | NM_001322470.2 | 30.5% | 29.3% | (many diffs) |
| 9 | human | 64799 | IQCH | IQ motif containing H | NM_001322475.2 | 28.8% | 27.7% | (many diffs) |
| 10 | human | 64799 | IQCH | IQ motif containing H | NM_001031715.3 | 21.4% | 20.6% | (many diffs) |
| 11 | human | 64799 | IQCH | IQ motif containing H | NR_147832.2 | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agtcaaaaca cctttgagag ccctgaaatc actgtgggat tatgactttt 121 taatttatga tggtgtcata gacaatacag ccccagactt cttagcattc aaggaacatt 181 ttagcttagc ttggggaggt attttttctc tcttggaaca cgtcgagaag tttctcagga 241 actatgctat accagaagtc aaaataaaag ggaataattt ggtggccctc cttccagagt 301 ttgagctgac gaataaactt accagatatg accTTCTCTC AGTGTTAGAG GACCCAGCTC 361 ATGTCCAAAT GCTGATAAAT CTTCCAGGGC AAAGGTACAA GGGCCAAGAT GGAAATTCGG 421 AGGCCGCCAT GAAGATCCAA GCCACATGGA AATGCTACAA AGCAAGAAAA TTCTTCCTCT 481 TTTATCGCCA GCAGAAGTGG GCATCAGGTG TGATTGCCAT TGCTTGGCTG TTATATTGCC 541 ATAAGACTCG ACTAAAGAAG ATACTAAAGG AATCACGTCA GAGACACCTG GAGAATTTTC 601 GCATTCGAGC CAAGCATCTG GCAGCCAACT GGAATCGCAT CAGGACCTCC AGGAGGACTA 661 TTATCCATAT CCCATCATTA GGTATAACAC TTTTGCCTGC AAATCTATCA GCAACCTCTA 721 TTACCCACCA CATTATGGCT CCTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AACTGTCTGT 901 AAAGCGAATC GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att