Construct: ORF TRCN0000469378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010726.1_s317c1
- Derived from:
- ccsbBroadEn_10694
- DNA Barcode:
- ATCGTGTGTTGTTGCAGTTCGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAT1 (586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 586 | BCAT1 | branched chain amino acid t... | NM_001178094.2 | 80.3% | 78.4% | (many diffs) |
| 2 | human | 586 | BCAT1 | branched chain amino acid t... | NM_005504.7 | 80% | 77.9% | (many diffs) |
| 3 | human | 586 | BCAT1 | branched chain amino acid t... | NM_001178093.2 | 77.5% | 75.6% | (many diffs) |
| 4 | human | 586 | BCAT1 | branched chain amino acid t... | NM_001178091.2 | 70.5% | 68.3% | (many diffs) |
| 5 | human | 586 | BCAT1 | branched chain amino acid t... | NM_001178092.2 | 64.7% | 62.5% | (many diffs) |
| 6 | human | 586 | BCAT1 | branched chain amino acid t... | XM_017019768.2 | 59.6% | 57.7% | (many diffs) |
| 7 | human | 586 | BCAT1 | branched chain amino acid t... | XR_001748835.2 | 10.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1026
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttgcagtaac ggatgctccg cagagtgtac cggagaagga ggatcaaaag 121 aggtggtggg gacttttaag gctaaagacc taatagtcac accagctacc attttaaagg 181 aaaaaccaga ccccaataat ctggtttttg gaactgtgtt cacggatcat atgctgacgg 241 tggagtggtc ctcagagttt ggatgggaga aacctcatat caagcctctt cagaacctgt 301 cattgcaccc tggctcatca gctttgcact atgcagtgga attatttgaa ggattgaagg 361 catttcgagg agtagataat aaaattcgac tgtttcagcc aaacctcaac atggatagaa 421 tgtatcgctc tgctgtgagg gcaactctgc cggtatttga caaagaagag ctcttagagt 481 gtattcaaca gcttgtgaaa ttggatcaag aatgggtccc atattcaaca tctgctagtc 541 tgtatattcg tcctacattc attggaactg agccttctct tggagtcaag aagcctacca 601 aagccctgct ctttgtactc ttgagcccag tgggacctta tttttcaagt ggaaccttta 661 atccagtgtc cctgtgggcc aaTCCCAAGT ATGTAAGAGC CTGGAAAGGT GGAACTGGGG 721 ACTGCAAGAT GGGAGGGAAT TACGGCTCAT CTCTTTTTGC CCAATGTGAA GCAGTAGATA 781 ATGGGTGTCA GCAGGTCCTG TGGCTCTATG GAGAGGACCA TCAGATCACT GAAGTGGGAA 841 CTATGAATCT TTTTCTTTAC TGGATAAATG AAGATGGAGA AGAAGAACTG GCAACTCCTC 901 CACTAGATGG CATCATTCTT CCAGGAGTGA CAAGGCGGTG CATTCTGGAC CTGGCACATC 961 AGTGGGACAC AGAACTCAGC TTGTTTTCAA TTAATTTGCC TGATTTTCTG CAGTTCATTT 1021 ACTTTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCGTGTGT TGTTGCAGTT CGAGCACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt