Construct: ORF TRCN0000469382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011648.1_s317c1
- Derived from:
- ccsbBroadEn_03695
- DNA Barcode:
- TTTTGAACAGGCTCTTCGGCTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXF2 (56001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 728343 | NXF2B | nuclear RNA export factor 2B | NM_001099686.2 | 99.9% | 100% | 1857G>A |
| 2 | human | 56001 | NXF2 | nuclear RNA export factor 2 | NM_022053.3 | 99.9% | 100% | 1857G>A |
| 3 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159739.1 | 74.7% | (many diffs) | |
| 4 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_028089.1 | 74.3% | (many diffs) | |
| 5 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159738.1 | 71.8% | (many diffs) | |
| 6 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159737.1 | 66.4% | (many diffs) | |
| 7 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159736.1 | 64.1% | (many diffs) | |
| 8 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NM_032946.2 | 54.7% | 50.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1944
- ORF length:
- 1878
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ctctactcta aagaagtgtg ggacatacag aactgaagtt gcagaatgcc 121 atgaccatgg tagcactttt caaggaagaa agaaaggtgg gagttctttc cgggataatt 181 ttgacaagag gagctgtcat tatgaacatg gtgggtatga gcgcccgcct tcacactgcc 241 aggagaatga tggaagcgtg gagatgaggg atgtccacaa ggaccaacaa ctaagacaca 301 ctccttatag catccgatgc gaaagaagaa tgaaatggca tagtgaagac gaaatccgta 361 ttaccacgtg gagaaataga aaacctccgg agagaaaaat gagtcagaac acacaggatg 421 gatacacaag gaactggttt aaggtcacaa ttccttacgg gataaagtat gacaaggcat 481 ggctaatgaa ttcaatccag agccattgca gtgaccgctt cactccggtt gatttccact 541 acgtccgaaa tcgggcatgc ttctttgtcc aggatgctag cgctgcctcc gcattgaagg 601 atgtcagtta taagatttat gatgatgaga accaaaagat atgtatattt gtcaatcatt 661 ctactgcgcc ctactctgtg aagaataagt tgaagccagg ccaaatggag atgctaaagc 721 tgaccatgaa caaacggtac aatgtctccc agcaagctct tgatctccag aatctccgct 781 ttgacccaga cttgatgggc cgtgacattg atataatcct gaatcgaaga aactgcatgg 841 ctgccaccct gaagatcatt gaaagaaatt tccctgagct gttgtctttg aacttgtgca 901 acaacaagct gtaccagctg gatggccttt ctgacattac agagaaggct cccaaagtca 961 agaccctgaa tctctccaaa aataagctgg agtcggcgtg ggagttgggc aaggtgaaag 1021 ggctgaagct cgaagagcta tggctagaag ggaacccgtt gtgcagcacc ttctcggacc 1081 agtccgccta tgtaagtgcc atccgggatt gtttccccaa gttgttacgc ctggacggcc 1141 gagagttatc cgcaccagtg attgttgaca ttgacagctc tgagacaatg aaaccctgca 1201 aggaaaactt tactggatct gagaccctaa agcatttagt cctgcaattc ctgcagcagt 1261 attactcgat ctatgactct ggagatcgac agggtctcct cggtgcttac cacgatgagg 1321 cctgcttctc cttggctatt cccttcgacc ccaaggactc agccccgagc agcttgtgca 1381 agtactttga ggatagcagg aatatgaaaa cactcaagga cccctacctg aagggggaac 1441 tgctgaggcg cacaaaacgt gacattgtgg actccctcag tgcgttgccc aaaactcagc 1501 atgaccTCAG CTCCATCCTG GTGGACGTGT GGTGCCAGAC GGAAAGGATG CTCTGCTTTT 1561 CTGTCAATGG GGTTTTCAAG GAAGTGGAAG GACAGTCTCA GGGTTCTGTT CTCGCCTTCA 1621 CCCGGACCTT CATTGCTACC CCTGGCAGCA GTTCCAGTCT GTGCATCGTG AATGACGAGC 1681 TGTTTGTGAG GGATGCCAGC CCCCAAGAGA CTCAGAGTGC CTTCTCCATC CCAGTGTCCA 1741 CACTCTCCTC CAGCTCTGAG CCCTCCCTCT CCCAGGAGCA GCAGGAAATG GTGCAGGCTT 1801 TCTCTGCCCA GTCTGGGATG AAACTGGAGT GGTCTCAGAA GTGCCTTCAG GACAATGAGT 1861 GGAACTACAC TAGAGCTGGC CAGGCCTTCA CTATGCTCCA GACCGAGGGC AAGATCCCCG 1921 CAGAGGCCTT CAAGCAAATC TCCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1981 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2041 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTTGAACAGG 2101 CTCTTCGGCT TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2161 att