Construct: ORF TRCN0000469382

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011648.1_s317c1
Derived from:
ccsbBroadEn_03695
DNA Barcode:
TTTTGAACAGGCTCTTCGGCTTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NXF2 (56001)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469382
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 728343 NXF2B nuclear RNA export factor 2B NM_001099686.2 99.9% 100% 1857G>A
2 human 56001 NXF2 nuclear RNA export factor 2 NM_022053.3 99.9% 100% 1857G>A
3 human 55998 NXF5 nuclear RNA export factor 5 NR_159739.1 74.7% (many diffs)
4 human 55998 NXF5 nuclear RNA export factor 5 NR_028089.1 74.3% (many diffs)
5 human 55998 NXF5 nuclear RNA export factor 5 NR_159738.1 71.8% (many diffs)
6 human 55998 NXF5 nuclear RNA export factor 5 NR_159737.1 66.4% (many diffs)
7 human 55998 NXF5 nuclear RNA export factor 5 NR_159736.1 64.1% (many diffs)
8 human 55998 NXF5 nuclear RNA export factor 5 NM_032946.2 54.7% 50.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1944
ORF length:
1878
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg ctctactcta aagaagtgtg ggacatacag aactgaagtt gcagaatgcc
121atgaccatgg tagcactttt caaggaagaa agaaaggtgg gagttctttc cgggataatt
181ttgacaagag gagctgtcat tatgaacatg gtgggtatga gcgcccgcct tcacactgcc
241aggagaatga tggaagcgtg gagatgaggg atgtccacaa ggaccaacaa ctaagacaca
301ctccttatag catccgatgc gaaagaagaa tgaaatggca tagtgaagac gaaatccgta
361ttaccacgtg gagaaataga aaacctccgg agagaaaaat gagtcagaac acacaggatg
421gatacacaag gaactggttt aaggtcacaa ttccttacgg gataaagtat gacaaggcat
481ggctaatgaa ttcaatccag agccattgca gtgaccgctt cactccggtt gatttccact
541acgtccgaaa tcgggcatgc ttctttgtcc aggatgctag cgctgcctcc gcattgaagg
601atgtcagtta taagatttat gatgatgaga accaaaagat atgtatattt gtcaatcatt
661ctactgcgcc ctactctgtg aagaataagt tgaagccagg ccaaatggag atgctaaagc
721tgaccatgaa caaacggtac aatgtctccc agcaagctct tgatctccag aatctccgct
781ttgacccaga cttgatgggc cgtgacattg atataatcct gaatcgaaga aactgcatgg
841ctgccaccct gaagatcatt gaaagaaatt tccctgagct gttgtctttg aacttgtgca
901acaacaagct gtaccagctg gatggccttt ctgacattac agagaaggct cccaaagtca
961agaccctgaa tctctccaaa aataagctgg agtcggcgtg ggagttgggc aaggtgaaag
1021ggctgaagct cgaagagcta tggctagaag ggaacccgtt gtgcagcacc ttctcggacc
1081agtccgccta tgtaagtgcc atccgggatt gtttccccaa gttgttacgc ctggacggcc
1141gagagttatc cgcaccagtg attgttgaca ttgacagctc tgagacaatg aaaccctgca
1201aggaaaactt tactggatct gagaccctaa agcatttagt cctgcaattc ctgcagcagt
1261attactcgat ctatgactct ggagatcgac agggtctcct cggtgcttac cacgatgagg
1321cctgcttctc cttggctatt cccttcgacc ccaaggactc agccccgagc agcttgtgca
1381agtactttga ggatagcagg aatatgaaaa cactcaagga cccctacctg aagggggaac
1441tgctgaggcg cacaaaacgt gacattgtgg actccctcag tgcgttgccc aaaactcagc
1501atgaccTCAG CTCCATCCTG GTGGACGTGT GGTGCCAGAC GGAAAGGATG CTCTGCTTTT
1561CTGTCAATGG GGTTTTCAAG GAAGTGGAAG GACAGTCTCA GGGTTCTGTT CTCGCCTTCA
1621CCCGGACCTT CATTGCTACC CCTGGCAGCA GTTCCAGTCT GTGCATCGTG AATGACGAGC
1681TGTTTGTGAG GGATGCCAGC CCCCAAGAGA CTCAGAGTGC CTTCTCCATC CCAGTGTCCA
1741CACTCTCCTC CAGCTCTGAG CCCTCCCTCT CCCAGGAGCA GCAGGAAATG GTGCAGGCTT
1801TCTCTGCCCA GTCTGGGATG AAACTGGAGT GGTCTCAGAA GTGCCTTCAG GACAATGAGT
1861GGAACTACAC TAGAGCTGGC CAGGCCTTCA CTATGCTCCA GACCGAGGGC AAGATCCCCG
1921CAGAGGCCTT CAAGCAAATC TCCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1981AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2041TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTTGAACAGG
2101CTCTTCGGCT TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2161att

Download FASTA (ORF) (Full)