Construct: ORF TRCN0000469384

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017518.1_s317c1
Derived from:
ccsbBroadEn_11824
DNA Barcode:
GCCAACAGCCATTGATCTCCGGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HIBCH (26275)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469384
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26275 HIBCH 3-hydroxyisobutyryl-CoA hyd... NM_198047.3 98.4% 98.2% 1_15del;136A>G
2 human 26275 HIBCH 3-hydroxyisobutyryl-CoA hyd... NM_014362.4 86.1% 85.7% (many diffs)
3 human 26275 HIBCH 3-hydroxyisobutyryl-CoA hyd... XM_011510953.2 86.1% 85.7% (many diffs)
4 human 26275 HIBCH 3-hydroxyisobutyryl-CoA hyd... XR_922903.2 72.5% 1_78del;199A>G;1078_1376del
5 human 26275 HIBCH 3-hydroxyisobutyryl-CoA hyd... XM_011510954.1 45.1% 44.8% 0_1ins483;514_525del;529_660del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1065
ORF length:
999
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg gaggctcatg tcgaggttta atgcattcaa aaggactaat accatactgc
121accatttgag aatgtccaag cacacagatg cagcagaaga ggtgctattg gaaaaaaaag
181gttgcgcggg agtcataaca ctaaacagac caaagttcct caatgcactg actcttaata
241tgattcggca gatttatcca cagctaaaga agtgggaaca agatcctgaa actttcctga
301tcattataaa gggagcagga ggaaaggctt tctgtgccgg gggtgatatc agagtgatct
361cggaagctga aaaggcaaaa cagaagatag ctccagtttt cttcagagaa gaatatatgc
421tgaataatgc tgttggttct tgccagaaac cttatgttgc acttattcat ggaattacaa
481tgggtggggg agttggtctc tcagtccatg ggcaatttcg agtggctaca gaaaagtgtc
541tttttgctat gccagaaact gcaataggac tgttccctga tgtgggtgga ggttatttct
601tgccacgact ccaaggaaaa cttggttact tccttgcatt aacaggattc agactaaaag
661gaagagatgt gtacagagca ggaattgcta cacactttgt agattctgaa aagttGGCCA
721TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT GCATCTGTCT
781TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA CTTGAGGAAC
841ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT ATTGAAAACT
901TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT AAAATGTCTC
961CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG ACCTTGCAAG
1021AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTACCCA ACTTTCTTGT
1081ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1141AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1201GAAAGGACGA GCCAACAGCC ATTGATCTCC GGAAACGCGT TAAGTCgaca atcaacctct
1261ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)