Construct: ORF TRCN0000469384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017518.1_s317c1
- Derived from:
- ccsbBroadEn_11824
- DNA Barcode:
- GCCAACAGCCATTGATCTCCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HIBCH (26275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26275 | HIBCH | 3-hydroxyisobutyryl-CoA hyd... | NM_198047.3 | 98.4% | 98.2% | 1_15del;136A>G |
| 2 | human | 26275 | HIBCH | 3-hydroxyisobutyryl-CoA hyd... | NM_014362.4 | 86.1% | 85.7% | (many diffs) |
| 3 | human | 26275 | HIBCH | 3-hydroxyisobutyryl-CoA hyd... | XM_011510953.2 | 86.1% | 85.7% | (many diffs) |
| 4 | human | 26275 | HIBCH | 3-hydroxyisobutyryl-CoA hyd... | XR_922903.2 | 72.5% | 1_78del;199A>G;1078_1376del | |
| 5 | human | 26275 | HIBCH | 3-hydroxyisobutyryl-CoA hyd... | XM_011510954.1 | 45.1% | 44.8% | 0_1ins483;514_525del;529_660del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaggctcatg tcgaggttta atgcattcaa aaggactaat accatactgc 121 accatttgag aatgtccaag cacacagatg cagcagaaga ggtgctattg gaaaaaaaag 181 gttgcgcggg agtcataaca ctaaacagac caaagttcct caatgcactg actcttaata 241 tgattcggca gatttatcca cagctaaaga agtgggaaca agatcctgaa actttcctga 301 tcattataaa gggagcagga ggaaaggctt tctgtgccgg gggtgatatc agagtgatct 361 cggaagctga aaaggcaaaa cagaagatag ctccagtttt cttcagagaa gaatatatgc 421 tgaataatgc tgttggttct tgccagaaac cttatgttgc acttattcat ggaattacaa 481 tgggtggggg agttggtctc tcagtccatg ggcaatttcg agtggctaca gaaaagtgtc 541 tttttgctat gccagaaact gcaataggac tgttccctga tgtgggtgga ggttatttct 601 tgccacgact ccaaggaaaa cttggttact tccttgcatt aacaggattc agactaaaag 661 gaagagatgt gtacagagca ggaattgcta cacactttgt agattctgaa aagttGGCCA 721 TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT GCATCTGTCT 781 TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA CTTGAGGAAC 841 ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT ATTGAAAACT 901 TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT AAAATGTCTC 961 CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG ACCTTGCAAG 1021 AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTACCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA GCCAACAGCC ATTGATCTCC GGAAACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt