Transcript: Human XM_011510953.2

PREDICTED: Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HIBCH (26275)
Length:
1410
CDS:
64..1224

Additional Resources:

NCBI RefSeq record:
XM_011510953.2
NBCI Gene record:
HIBCH (26275)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011510953.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000078607 CCTAGAGCAATTGAAGGTAAT pLKO.1 939 CDS 100% 10.800 8.640 N HIBCH n/a
2 TRCN0000229744 TGATGTGGGTGGAGGTTATTT pLKO_005 591 CDS 100% 15.000 10.500 N HIBCH n/a
3 TRCN0000251113 TGATGTGGGTGGAGGTTATTT pLKO_005 591 CDS 100% 15.000 10.500 N Hibch n/a
4 TRCN0000229742 TAATATGATTCGGCAGATTTA pLKO_005 249 CDS 100% 13.200 9.240 N HIBCH n/a
5 TRCN0000229743 TGAAACTTTCCTGATCATTAT pLKO_005 300 CDS 100% 13.200 9.240 N HIBCH n/a
6 TRCN0000078606 CTTAATATGATTCGGCAGATT pLKO.1 247 CDS 100% 4.950 3.465 N HIBCH n/a
7 TRCN0000078605 CCACAGCTAAAGAAGTGGGAA pLKO.1 271 CDS 100% 2.640 1.848 N HIBCH n/a
8 TRCN0000078604 GCACCATTTGAGAATGTCCAA pLKO.1 132 CDS 100% 2.640 1.848 N HIBCH n/a
9 TRCN0000229745 ATGGAAACCAGCTGATCTAAA pLKO_005 1137 CDS 100% 13.200 7.920 N HIBCH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011510953.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11824 pDONR223 100% 86.1% 85.7% None (many diffs) n/a
2 ccsbBroad304_11824 pLX_304 0% 86.1% 85.7% V5 (many diffs) n/a
3 TRCN0000469384 GCCAACAGCCATTGATCTCCGGAA pLX_317 35.3% 86.1% 85.7% V5 (many diffs) n/a
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