Construct: ORF TRCN0000469393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018323.1_s317c1
- Derived from:
- ccsbBroadEn_13511
- DNA Barcode:
- CCAATATCAGGCCCAGGCCGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDRT4 (284040)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284040 | CDRT4 | CMT1A duplicated region tra... | NM_001204477.2 | 96.7% | 96.7% | 1_15del |
| 2 | human | 100533496 | TVP23C-CDRT4 | TVP23C-CDRT4 readthrough | NR_037924.2 | 16.8% | 1_414del;856_2616del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaaagaagaa ggactcacag aaaacactgg acttccccgg aagctacttg 121 aaaaacatga cccctggccg gcctatgtca cctataccTC TCAGACAGTG AAAAGACTCA 181 TTGAGAAAAG CAAAACTAGA GAACTGGAAT GCATGCGTGC CCTCGAGGAA AGACCCTGGG 241 CATCAAGGCA GAATAAACCT TCCAGCGTCA TTCAGCCGAA AAGGAGGAAG TCTTCCAAGT 301 CTTCTGGCAA AGCTGTGTTC AGGGACACGC TGTCAGAATC CACGTTATCA ATGTGGGGCG 361 CTTATTCGGT ATTGGCCATG GCTCCTACCA TGATCCCAGA ACCCACACAC TTACATGCGG 421 ATTCCAGAGA CTGTCCAACT GAAAACTATA ACAAGATCAT CTTTGCCCGC AAGCCTATGA 481 TGAGGATGCT CCCTACAGTT CGCTACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCAATATC 661 AGGCCCAGGC CGGCGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt