Construct: ORF TRCN0000469409
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008615.1_s317c1
- Derived from:
- ccsbBroadEn_11613
- DNA Barcode:
- TTGTTTTCAGCGAGCACACGTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKAP13 (11214)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469409
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11214 | AKAP13 | A-kinase anchoring protein 13 | NM_007200.5 | 6% | 5.7% | (many diffs) |
| 2 | human | 11214 | AKAP13 | A-kinase anchoring protein 13 | NM_006738.6 | 6% | 5.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acttaatcca cagcaagctc ccttatatgg tgattgtgtt gttacagtgc 121 tgcttgctga agaggacaaa gctgaagatg atgtagtgtt ttacttggta tttttgggtt 181 ccaccctccg tcactgtaca agtactcgga aggtcagttc TGATACATTG GAGACCATTG 241 CTCCTGGTCA TGATTGTTGT GAAACAGTGA AGGTGCAGCT CTGTGCTTCC AAAGAGGGCC 301 TTCCCGTGTT TGTGGTGGCT GAAGAAGACT TTCATTTCGT CCAGGATGAA GCGTATGATG 361 CAGCTCAATT CCTAGCAACC AGTGCTGGAA ATCAGCAGGC TTTGAACTTT ACCCGTTTTC 421 TTGACCAGTC AGGACCCCCA TCTGGGGATG TGAATTCCCT TGATAAGAAG TTGGTGCTGG 481 CATTCAGGCA CCTGAAGCTG CCCACGGAGT GGAATGTATT GGGGACAGAT CAGAGTTTGC 541 ATGGTGAGAA TTTATATGAT CTACAAACAC ACTTTAAGTT TGTGATATTT CTACTTTTTT 601 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGATT GTTTTCAGCG AGCACACGTT AGACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt