Construct: ORF TRCN0000469416
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018027.1_s317c1
- Derived from:
- ccsbBroadEn_04335
- DNA Barcode:
- AATACAAAACTCCCATCACTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EVA1A (84141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469416
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001135032.2 | 100% | 100% | |
| 2 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001369524.1 | 100% | 100% | |
| 3 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001369525.1 | 100% | 100% | |
| 4 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_032181.3 | 100% | 100% | |
| 5 | mouse | 232146 | Eva1a | eva-1 homolog A (C. elegans) | NM_145570.2 | 82.3% | 84.7% | (many diffs) |
| 6 | mouse | 232146 | Eva1a | eva-1 homolog A (C. elegans) | XM_006505962.1 | 82.3% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gctgccccTC AGCCACAGCC CAGAGCACGT GGAGATGGCT TTGCTCAGCA 121 ACATCCTAGC GGCCTATTCC TTTGTCTCAG AAAATCCTGA GCGAGCAGCT CTGTACTTTG 181 TTTCTGGCGT GTGCATCGGG CTGGTGCTGA CCCTGGCTGC TCTGGTGATA AGGATCTCTT 241 GCCACACAGA CTGCAGGCGG CGTCCCGGGA AGAAGTTCCT GCAGGACAGA GAGAGCAGCA 301 GCGACAGCAG CGACAGCGAG GATGGCAGTG AGGACACCGT GTCCGATCTC TCCGTGCGGA 361 GACACCGCCG CTTCGAGAGG ACTTTGAACA AGAATGTGTT CACCTCTGCG GAGGAGCTGG 421 AGCGCGCCCA GCGGCTGGAG GAGCGCGAGC GCATCATCAG GGAGATCTGG ATGAATGGCC 481 AGCCTGAGGT GCCCGGGACC AGGAGCCTGA ATCGCTACTA TTACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAAAT ACAAAACTCC CATCACTTGA CACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t