Construct: ORF TRCN0000469426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009600.1_s317c1
- Derived from:
- ccsbBroadEn_05419
- DNA Barcode:
- ACGATACCACTCTATACAGACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM59 (286827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286827 | TRIM59 | tripartite motif containing 59 | NM_173084.3 | 100% | 100% | |
| 2 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148401.1 | 33.4% | (many diffs) | |
| 3 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148402.1 | 17.9% | (many diffs) | |
| 4 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148403.1 | 17.2% | (many diffs) | |
| 5 | mouse | 66949 | Trim59 | tripartite motif-containing 59 | NM_025863.3 | 85.7% | 82.6% | (many diffs) |
| 6 | mouse | 66949 | Trim59 | tripartite motif-containing 59 | XM_006501917.3 | 85.7% | 82.6% | (many diffs) |
| 7 | mouse | 66949 | Trim59 | tripartite motif-containing 59 | XM_011240200.1 | 85.7% | 82.6% | (many diffs) |
| 8 | mouse | 66949 | Trim59 | tripartite motif-containing 59 | XM_011240201.2 | 85.7% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1278
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacaatttt gaggaagagt taacttgtcc catatgttat agtatttttg 121 aagatcctcg tgtactgcca tgctctcata cattttgtag aaattgtttg gaaaacattc 181 ttcaggcatc tggtaacttt tatatatgga gacctttacg aattccactc aagtgcccta 241 attgcagaag tattactgaa attgctccaa ctggcattga atctttacct gttaattttg 301 cactaagggc tattattgaa aagtaccagc aagaagacca tccagatatt gtcacctgcc 361 ctgaacatta caggcaacca ttaaatgttt actgtctatt agataaaaaa ttagtttgtg 421 gtcattgcct taccataggt caacatcatg gtcatcctat agatgacctt caaagtgcct 481 atttgaaaga aaaggacact cctcaaaaac tgcttgaaca gttgactgac acacactgga 541 cagatcttac ccatcttatt gaaaagctga aagaacaaaa atctcattct gagaaaatga 601 tccaaggcga taaggaagct gttctccagt attttaagga gcttaatgat acattagaac 661 agaaaaaaaa aagtttccta acggctctct gtgatgttgg caatctaatt aatcaagaat 721 atactccaca aattgaaaga atgaaggaaa tacgagagca gcagcttgaa ttaatggcac 781 tgacaatatc tttacaagaa gagtctccac ttaaatttct tgaaaaagtt gatgatgtac 841 gccagcatgt acagatcttg aaacaaagac cacttccTGA GGTTCAACCC GTTGAAATTT 901 ATCCTCGAGT AAGCAAAATA TTGAAAGAAG AATGGAGCAG AACAGAAATT GGACAAATTA 961 AGAACGTTCT CATTCCCAAA ATGAAAATTT CTCCAAAAAG GATGTCATGT TCCTGGCCTG 1021 GTAAGGATGA AAAGGAAGTT GAATTTTTAA AAATTTTAAA CATTGTTGTA GTTACATTAA 1081 TTTCAGTAAT ACTGATGTCG ATACTCTTTT TCAACCAACA CATCATAACC TTTTTAAGTG 1141 AAATCACTTT AATATGGTTT TCTGAAGCCT CTCTATCTGT TTACCAAAGT TTATCTAACA 1201 GTCTGCATAA GGTAAAGAAT ATACTGTGTC ACATTTTCTA TTTGTTGAAG GAATTTGTGT 1261 GGAAAATAGT TTCCCATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGATAC CACTCTATAC 1441 AGACCATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt