Construct: ORF TRCN0000469429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006561.1_s317c1
- Derived from:
- ccsbBroadEn_03185
- DNA Barcode:
- CGTTCTTTAAATAGAGTGTCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PI15 (51050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51050 | PI15 | peptidase inhibitor 15 | NM_001324403.2 | 100% | 100% | |
| 2 | human | 51050 | PI15 | peptidase inhibitor 15 | NM_015886.5 | 100% | 100% | |
| 3 | mouse | 94227 | Pi15 | peptidase inhibitor 15 | NM_053191.2 | 84% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 843
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatagcaatc tctgccgtca gcagtgcact cctgttctcc cttctctgtg 121 aagcaagtac cgtcgtccta ctcaattcca ctgactcatc cccgccaacc aataatttca 181 ctgatattga agcagctctg aaagcacaat tagattcagc ggatatcccc aaagccaggc 241 ggaagcgcta catttcgcag aatgacatga tcgccattct tgattatcat aatcaagttc 301 ggggcaaagt gttcccaccg gcagcaaata tggaatatat ggtttgggat gaaaatcttg 361 caaaatcggc agaggcttGG GCGGCTACTT GCATTTGGGA CCATGGACCT TCTTACTTAC 421 TGAGATTTTT GGGCCAAAAT CTATCTGTAC GCACTGGAAG ATATCGCTCT ATTCTCCAGT 481 TGGTCAAGCC ATGGTATGAT GAAGTGAAAG ATTATGCTTT TCCATATCCC CAGGATTGCA 541 ACCCCAGATG TCCTATGAGA TGTTTTGGTC CCATGTGCAC ACATTATACG CAGATGGTTT 601 GGGCCACTTC CAATCGGATA GGATGCGCAA TTCATACTTG CCAAAACATG AATGTTTGGG 661 GATCTGTGTG GCGACGTGCA GTTTACTTGG TATGCAACTA TGCCCCAAAG GGCAATTGGA 721 TTGGAGAAGC ACCATATAAA GTAGGGGTAC CATGTTCATC TTGTCCTCCA AGTTATGGGG 781 GATCTTGTAC TGACAATCTG TGTTTTCCAG GAGTTACGTC AAACTACCTG TACTGGTTTA 841 AATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACG TTCTTTAAAT AGAGTGTCTA CGACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt