Construct: ORF TRCN0000469431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015663.1_s317c1
- Derived from:
- ccsbBroadEn_04849
- DNA Barcode:
- CGACTCTTGCTAGTTCCGAGAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR1-1HG (128826)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128826 | MIR1-1HG | MIR1-1 host gene | NM_001302812.2 | 100% | 100% | |
| 2 | human | 128826 | MIR1-1HG | MIR1-1 host gene | NM_178463.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 444
- ORF length:
- 351
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCCCAG CTGTAGCTGT GCATTGATGG 121 CGCCGTGCGG CCCGGCCGCA GGTCCCGCAG CCGTGGAGAG GACCCAGCAG GTGGCGCGGG 181 GAGAGCCCGG CTCGGCACGT GGTCAGCTCC AAGTCAGCCC TGAAATGTCC ATTACTCACA 241 AAGAAAAGGA AAATGCTCAT TTGAAAGAAA TTCTTCTCTT TGTCAATGCT GAGGCATTCT 301 CTCAGCCCCA GCCCCACAGT GCTCCAGTGT GTGAAGGGCA ACAGCTGACG GGGAAGTTCA 361 GCACATCTGT GCTGACCAGG GCTGGAGGCG ATGCCAGTCC GTGCAGCTGG GAACGCCTCC 421 TCTGCTATGG CTGGAGCCAC TGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GACTCTTGCT 601 AGTTCCGAGA GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att