Construct: ORF TRCN0000469437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010751.1_s317c1
- Derived from:
- ccsbBroadEn_01231
- DNA Barcode:
- GACGGCAACTTCGGGAATCACTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNMT (5409)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5409 | PNMT | phenylethanolamine N-methyl... | NM_002686.4 | 100% | 100% | |
| 2 | human | 5409 | PNMT | phenylethanolamine N-methyl... | NR_073461.2 | 72.4% | (many diffs) | |
| 3 | human | 5409 | PNMT | phenylethanolamine N-methyl... | XM_011524909.2 | 65.2% | 65.2% | 0_1ins294 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcgcagac cgtagcccca atgcgggcgc agcccctgac tcggccccgg 121 gccaggcggc ggtggcttcg gcctaccagc gcttcgagcc gcgcgcctac ctccgcaaca 181 actacgcgcc ccctcgcggg gacctgtgca acccgaacgg cgtcgggccg tggaagctgc 241 gctgcttggc gcagaccttc gccaccggtg aagtgtccgg acgcaccctc atcgacattg 301 gttcaggccc caccgtgtac cagctgctca gtgcctgcag ccactttgag gacatcacca 361 tgacagattt cctggaggtc aaccgccagg agctggggcg ctggctgcag gaggagccgg 421 gggccttcaa ctggagcatg tacagccaac atgcctgcct CATTGAGGGC AAGGGGGAAT 481 GCTGGCAGGA TAAGGAGCGC CAGCTGCGAG CCAGGGTGAA ACGGGTCCTG CCCATCGACG 541 TGCACCAGCC CCAGCCCCTG GGTGCTGGGA GCCCAGCTCC CCTGCCTGCT GACGCCCTGG 601 TCTCTGCCTT CTGCTTGGAG GCTGTGAGCC CAGATCTTGC CAGCTTTCAG CGGGCCCTGG 661 ACCACATCAC CACGCTGCTG AGGCCTGGGG GGCACCTCCT CCTCATCGGG GCCCTGGAGG 721 AGTCGTGGTA CCTGGCTGGG GAGGCCAGGC TGACGGTGGT GCCAGTGTCT GAGGAGGAGG 781 TGAGGGAGGC CCTGGTGCGT AGTGGCTACA AGGTCCGGGA CCTCCGCACC TATATCATGC 841 CTGCCCACCT TCAGACAGGC GTAGATGATG TCAAGGGCGT CTTCTTCGCC TGGGCTCAGA 901 AGGTTGGGCT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAC GGCAACTTCG GGAATCACTA 1081 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t