Construct: ORF TRCN0000469447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003106.1_s317c1
- Derived from:
- ccsbBroadEn_11999
- DNA Barcode:
- TTGCCGAACCGCGCACTCAGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIAS4 (51588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51588 | PIAS4 | protein inhibitor of activa... | NM_015897.4 | 97.9% | 98% | 1_30del;738C>T;1026T>C |
| 2 | human | 51588 | PIAS4 | protein inhibitor of activa... | XM_011528060.2 | 94.3% | 94.5% | 1_87del;795C>T;1083T>C |
| 3 | human | 51588 | PIAS4 | protein inhibitor of activa... | XM_017026868.1 | 63.6% | 63.4% | (many diffs) |
| 4 | mouse | 59004 | Pias4 | protein inhibitor of activa... | NM_021501.4 | 81% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gatgagtttt cgagtctccg accttcagat gctcctgggt ttcgtgggcc 121 ggagtaagag tggactgaag cacgagctcg tcaccagggc cctccagctg gtgcagtttg 181 actgtagccc tgagctgttc aagaagatca aggagctgta cgagacccgc tacgccaaga 241 agaactcgga gcctgcccca cagccgcacc ggcccctgga ccccctgacc atgcactcca 301 cctacgaccg ggccggcgct gtgcccagga ctccgctggc aggccccaat attgactacc 361 ccgtgctcta cggaaagtac ttaaacggac tgggacggtt gcccgccaag accctcaagc 421 cagaagtccg cctggtgaag ctgccgttct ttaatatgct ggatgagctg ctgaagccca 481 ccgaattagt cccacagaac aacgagaagc ttcaggagag cccgtgcatc ttcgcattga 541 cgccaagaca ggtggagttg atccggaact ccagggaact gcagcccgga gttaaagccg 601 tgcaggtcgt cctgagaatc tgttactcag acaccagctg ccctcaggag gaccagtacc 661 cgcccaacat cgctgtgaag gtcaaccaca gctactgctc cgtcccgggc tactacccct 721 ccaataagcc cggggtggag cccaagaggc cgtgccgccc catcaacctc actcacctca 781 tgtacctgtc ctcggccacc aaccgcatca ctgtcacctg ggggaactac ggcaagagct 841 actcggtggc cctgtacctg gtgcggcagc tgacctcatc ggagctgctg cagaggctga 901 agaccattgg ggtaaagcac ccggagctgt gcaaggcact ggtcaaggag aagctgcgcc 961 ttgatcctga cagcgagatc gccaccaccg gtgtgcgggt gtccctcatc tgtccgctgg 1021 tgaagatgcg gctctccgtg ccctgccggg cagagacctg cgcccacctg cagtgcttcg 1081 acgccgtctt ctacctgcag atgaacgaga agaagcccac ctggatgtgc cccgtgtgcg 1141 acaagccagc ccccTACGAC CAGCTCATCA TCGACGGGCT CCTCTCGAAG ATCCTGAGCG 1201 AGTGTGAGGA CGCCGACGAG ATCGAGTACC TGGTGGACGG CTCGTGGTGC CCGATCCGCG 1261 CCGAAAAGGA GCGCAGCTGC AGCCCGCAGG GCGCCATCCT CGTGCTGGGC CCCTCGGACG 1321 CCAATGGGCT CCTGCCCGCC CCCAGCGTCA ACGGGAGCGG TGCCCTGGGC AGCACGGGTG 1381 GCGGCGGCCC GGTGGGCAGC ATGGAGAATG GGAAGCCGGG CGCCGATGTG GTGGACCTCA 1441 CGCTGGACAG CTCATCGTCC TCGGAGGATG AGGAGGAGGA GGAAGAGGAG GAGGAAGACG 1501 AGGACGAAGA GGGGCCCCGG CCCAAGCGCC GCTGCCCCTT CCAGAAGGGC CTGGTGCCGG 1561 CCTGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTGCCGAAC CGCGCACTCA GAATCACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt