Construct: ORF TRCN0000469476
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013365.1_s317c1
- Derived from:
- ccsbBroadEn_10866
- DNA Barcode:
- CTTCATGACTGGGGAGCTCTAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTA4 (2941)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469476
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2941 | GSTA4 | glutathione S-transferase a... | XM_011514534.3 | 69.7% | 69.7% | 1_168del |
| 2 | human | 2941 | GSTA4 | glutathione S-transferase a... | XM_011514535.3 | 69.7% | 69.7% | 1_168del |
| 3 | human | 2941 | GSTA4 | glutathione S-transferase a... | NM_001512.4 | 58.1% | 58.1% | 1_279del |
| 4 | human | 2941 | GSTA4 | glutathione S-transferase a... | XM_005249035.4 | 58.1% | 58.1% | 1_279del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGTA CGTGGAGGGG ACACTGGATC TGCTGGAACT GCTTATCATG CATCCTTTCT 121 TAAAACCAGA TGATCAGCAA AAGGAAGTGG TTAACATGGC CCAGAAGGCT ATAATTAGAT 181 ACTTTCCTGT GTTTGAAAAG ATTTTAAGGG GTCACGGACA AAGCTTTCTT GTTGGTAATC 241 AGCTGAGCCT TGCAGATGTG ATTTTACTCC AAACCATTTT AGCTCTAGAA GAGAAAATTC 301 CTAATATCCT GTCTGCATTT CCTTTCCTCC AGGAATACAC AGTGAAACTA AGTAATATCC 361 CTACAATTAA GAGATTCCTT GAACCTGGCA GCAAGAAGAA GCCTCCCCCT GATGAAATTT 421 ATGTGAGAAC CGTCTACAAC ATCTTTAGGC CATACCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 601 TCATGACTGG GGAGCTCTAG GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt