Construct: ORF TRCN0000469501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010335.1_s317c1
- Derived from:
- ccsbBroadEn_03895
- DNA Barcode:
- TCGGTACATAACTGGCTGAATAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCOC (60592)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 60592 | SCOC | short coiled-coil protein | NM_001153635.1 | 100% | 100% | |
2 | human | 60592 | SCOC | short coiled-coil protein | NM_001153446.1 | 99.1% | 99.1% | 142_144delGCA |
3 | human | 60592 | SCOC | short coiled-coil protein | NM_001153585.1 | 99.1% | 99.1% | 142_144delGCA |
4 | human | 60592 | SCOC | short coiled-coil protein | NM_032547.3 | 99.1% | 99.1% | 142_144delGCA |
5 | human | 60592 | SCOC | short coiled-coil protein | NM_001153690.1 | 77.6% | 77.6% | 141_142ins81 |
6 | human | 60592 | SCOC | short coiled-coil protein | NM_001153663.1 | 67.6% | 64.3% | (many diffs) |
7 | human | 60592 | SCOC | short coiled-coil protein | NM_001153484.1 | 67.2% | 63.9% | (many diffs) |
8 | human | 60592 | SCOC | short coiled-coil protein | NM_001153552.1 | 51% | 47.5% | (many diffs) |
9 | mouse | 56367 | Scoc | short coiled-coil protein | XM_011248448.3 | 86.5% | 85.4% | (many diffs) |
10 | mouse | 56367 | Scoc | short coiled-coil protein | NM_001039137.3 | 85.8% | 84.8% | (many diffs) |
11 | mouse | 56367 | Scoc | short coiled-coil protein | NM_001285992.1 | 62.5% | 66.9% | (many diffs) |
12 | mouse | 56367 | Scoc | short coiled-coil protein | XM_006531205.2 | 62.5% | 66.9% | (many diffs) |
13 | mouse | 56367 | Scoc | short coiled-coil protein | NM_019708.4 | 62% | 66.3% | (many diffs) |
14 | mouse | 56367 | Scoc | short coiled-coil protein | XM_006531204.4 | 62% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 453
- ORF length:
- 363
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGACGGGTC CAGGAAAGAG GAGGAGGAAG 121 ACAGCACATT CACCAACATT TCTCTTGCAG ATGACATAGA CCATTCCTCA AGAATTTTGT 181 ATCCAAGGCC CAAAAGTTTG TTACCCAAGA TGATGAATGC TGACATGGAT GTTGATGCTG 241 AAAATCAAGT GGAACTGGAG GAAAAAACAA GACTTATTAA TCAAGTGTTG GAACTCCAAC 301 ACACACTTGA AGATCTCTCT GCAAGAGTAG ATGCAGTTAA GGAAGAAAAT CTGAAGCTAA 361 AATCAGAAAA CCAAGTTCTT GGACAATATA TAGAAAATCT CATGTCAGCT TCTAGTGTTT 421 TTCAAACAAC TGACACAAAA AGCAAAAGAA AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 601 GGTACATAAC TGGCTGAATA AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt